Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an invitro study
Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an invitro study
复制标题
Biodentine 和 iRoot FS 与人牙周膜细胞的细胞相容性：体外研究
DOI：
10.1111/iej.12889
复制
发表时间：
2018-07-01
影响因子：
5
通讯作者：
Zou, L.
中科院分区：
文献类型：
--
作者：
Luo, T.;Liu, J.;Zou, L.
AimTo evaluate the cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells (hPDLCs).MethodologyHuman periodontal ligament cells were characterized by flow cytometry and immunocytochemical analysis. Human periodontal ligament cell adhesion was assessed by scanning electron microscopy at day 3; proliferation by live/dead assay at days 1, 3 and 7; and osteogenic differentiation by alkaline phosphatase (ALP) activity staining, ALP quantification analysis and qRT-PCR at days 7 and 14. Data were analysed with anova and independent sample t-tests with SPSS 21.0.ResultsBoth iRoot FS and Biodentine increased the adhesion of hPDLCs at day 3. Compared to Biodentine, iRoot FS positively increased hPDLC proliferation on days 3 (P=0.03) and 7 (P=0.00). Osteogenic marker ALP was observed consistently in all samples, with iRoot FS having significantly higher ALP activity at day 14 (P=0.00). Compared with Biodentine, iRoot FS significantly increased the mRNA level of ALP, COL1 and Runx2, and OCN increased only on day 14 (P
