Real-Time Monitoring of Circadian Rhythms in the Eye.
Real-Time Monitoring of Circadian Rhythms in the Eye.
复制标题
实时监测眼睛的昼夜节律。
DOI：
10.1007/978-1-0716-2593-4_37
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tosini,Gianluca
中科院分区：
文献类型：
--
作者：
Baba,Kenkichi;Tosini,Gianluca
哺乳动物的眼睛有一个完整的昼夜节律系统，控制着这个器官内几个生理上相关的功能。在过去的二十年里，一些实验室已经开发出转基因动物模型，在这种模型中，可以使用荧光素酶的活性来实时监测昼夜节律。最著名的记录不同组织器官生物发光节律的转基因小鼠是高桥实验室在2000年初培育的Perod2：：LUC(Perod2：：Luc)小鼠。从那时起，几项研究利用这种小鼠模型，通过监测大脑、眼睛和许多其他外围器官和组织的昼夜节律来剖析哺乳动物的昼夜节律系统。本章描述了记录和分析PER2：：Luc小鼠视网膜、视网膜色素上皮和角膜的生物发光节律的方法。
The mammalian eye harbors a full circadian system that controls several physiologically relevant functions within this organ. During the last two decades a few laboratories have developed transgenic animal models in which circadian rhythms can be monitored in real time using luciferase activity. The most famous transgenic mouse to record bioluminescence rhythms from different tissues and organs is the PERIOD2::LUCIFERASE (PER2::LUC) mouse developed by the Takahashi laboratory in early 2000. Since then, several studies have used this mouse model to dissect the mammalian circadian system by monitoring the circadian rhythm in the brain, the eye, and in many other peripheral organs and tissues. This chapter describes the methodology to record and analyze bioluminescence rhythms from the retina, retinal pigment epithelium, and cornea of PER2::LUC mice.