Lymphocyte Transformation
Lymphocyte Transformation
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淋巴细胞转化
DOI：
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发表时间：
1972
影响因子：
10.3
通讯作者：
S. Alexander
中科院分区：
文献类型：
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作者：
S. Alexander
SIB，-我们指的是淋巴细胞转化而不是胸苷吸收率，因为我们发现这两种测试测量的参数略有不同。通过计数染色胶片中的转化细胞来测量的“淋巴细胞转化“在我们手中被发现比胸苷摄取率更能提供信息。在染色的胶片中，细胞中的“毒性“变化可以与“母细胞转化“同时检测到，然后可以改变刺激剂的剂量。在胸苷摄取率中，这可能显示没有反应，因为（1）刺激剂的剂量太高，（2）太低，（3）毒性太大。没有关于这三种细胞中哪一种的信息，而通过更繁琐的计数和观察细胞的方法可以获得线索，因此有了这个术语。哈钦森先生、麦克劳德先生和莱佛士先生似乎担心我们没有采取足够数量的读数。句子“试验尽可能重复进行，使用直接法和。. .给培养物贴上标签。. .“意味着我们在有足够血液的情况下使用了两种方法，-也许“平行“是一个更好的表达-这当然不意味着当我们使用胸苷摄取方法时，我们只使用了一个等分试样：我们所有的测试，常规地，对所使用的每种药物强度进行3等份，事实上，当我们一式两份进行试验时，对于每种浓度的药物，我们有3份等分试样用于胸苷摄取，2份等分试样用于计数方法。图2中给出的每分钟的分解是这3个等分试样中每一个的平均读数，并且当一个阅读与其他读数的变化超过20%时，所有3个等分试样都被丢弃。
SIB,—We referred to lymphocyte transformation rather than thymidine uptake ratio because we have found that these 2 tests measure slightly different parameters. The " lymphocyte transformation " as measured by counting transformed cells in a stained film was found in our hands to be more informative than the thjrmidine uptake ratio. In a stained film " toxic " changes in the cells can be detected simultaneously with " blast cell transformation " and the dose of the stimulating agent can then be varied In the thymidine uptake ratio this may show no reaction because (1) The dose of the stimulating agent was too high, (2) too low, (3) too toxic. No information to which of the 3 is given while a clue can be obtained by the much more tedious methods of counting and looking at the cells—hence the terminology. Messrs Hutchinson, Macleod and Raffle appear to be worried that we did not take a sufficient number of readings. The sentence " tests were carried out in duplicate as far as was possible, using both the direct method and . . . that of labelling the cultures . . . ' means that we used both methods where we had enough blood,—perhaps " in parallel " would have been a better expression—it does certainly not mean that when we used the thymidine uptake method we only used one aliquot: all our tests, routinely, are done on 3 aliquots for each strength of drug used, and in fact when we did the test in " duplicate" we had 3 aliquots for each concentration of drug for the thymidine uptake and 2 for the counting method. The distintegrations per min given in Fig. 2 were the average readings of each of these 3 ahquots, and where one reading varied by more than 20% from the others, all 3 were discarded.