Effect of Amine Type on the Expression of Plasmid DNA by Cationized Dextran
Effect of Amine Type on the Expression of Plasmid DNA by Cationized Dextran
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DOI：
10.1163/156856209x415549
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Jo, Jun-ichiro;Nagane, Kentaro;Tabata, Yasuhiko
本研究的目的是从多糖葡聚糖制备基因表达的非病毒载体，并评估引入葡聚糖的胺化合物对基因表达水平的影响。分子量为74 × 10（3）的葡聚糖通过化学引入不同的胺化合物进行阳离子化。将阳离子化葡聚糖与质粒DNA复合，并对HeLa细胞进行体外基因转染研究。基因表达水平取决于葡聚糖中引入的胺化合物。最高水平的观察为精胺引入的葡聚糖和质粒DNA的复合物。在每种阳离子化葡聚糖中观察到最高的细胞内化和最好的缓冲效果。各复合物均未显示出任何细胞毒性。与其他阳离子葡聚糖相比，精胺引入葡聚糖的上级性质使质粒DNA在很大程度上提高了表达水平。阳离子葡聚糖是一种很有前途的质粒DNA非病毒载体。(C)Koninklijke Brill NV，莱顿，2010年
The objective of this study is to prepare a non-viral carrier of gene expression from the polysaccharide dextran and evaluate the effect of amine compounds introduced to dextran on the level of gene expression. Dextran with a molecular weight of 74 x 10(3) was cationized by the chemical introduction of different amine compounds. The cationized dextran was complexed with a plasmid DNA and the vitro gene transfection was investigated for HeLa cells. The level of gene expression depended on the amine compound introduced to dextran. The highest level was observed for the complex of spermine-introduced dextran and plasmid DNA. The highest cellular internalization and the best buffering effect were observed among every cationized dextran. Every complex did not show any cytotoxicity. It is concluded that the superior properties of spermine-introduced dextran enabled the plasmid DNA to enhance the expression level to a great extent compared with other cationized dextrans. Cationized dextran is a promising non-viral carrier of plasmid DNA. (C) Koninklijke Brill NV, Leiden, 2010