The role of ultraviolet‐adaptation of a marine diatom in photoenhanced toxicity of acridine
The role of ultraviolet‐adaptation of a marine diatom in photoenhanced toxicity of acridine
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DOI：
10.1002/etc.5620220318
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发表时间：
2003-03
影响因子：
4.1
通讯作者：
S. Wiegman;C. Barranguet;E. Spijkerman;M. Kraak;W. Admiraal
中科院分区：
文献类型：
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作者：
S. Wiegman;C. Barranguet;E. Spijkerman;M. Kraak;W. Admiraal
在不同比例紫外线照射下培养海洋硅藻褐藻（Phaeodactylum tricornutum），研究其光适应在测定吖啶光增强毒性敏感性中的潜在作用。在短期实验中，暴露在荧光灯（低紫外线）下的藻类比暴露在汞光（高紫外线）下的藻类需要更高的吖啶浓度来抑制光合电子通量，这与紫外线的预期作用一致。两种类型的光长期暴露导致色素组成和光系统I （PS I）向光系统II （PS II）化学计量的变化，以优化对荧光和汞光的利用。尽管光合机构适应了一小部分紫外线，但长期暴露于汞光下确实显示出三角角藻对光毒性吖啶的光合效率具有恒定的敏感性。因此，光增强毒性的主要受体可能与光合机制无关。
Cultures of the marine diatom Phaeodactylum tricornutum were grown under laboratory light with a different fraction of ultraviolet radiation (UV) to study the potential role of photoadaptation in determining the sensitivity to photoenhanced toxicity of acridine. In short‐term experiments, a higher acridine concentration was needed to inhibit the photosynthetic electron flux, monitored with chlorophyll a fluorescence, in algae exposed to fluorescent light (low UV) than to mercury light (high UV), consistent with the expected role of UV. The two types of light in long‐term exposures led to changes in the pigment composition and photosystem I (PS I) to photosystem II (PS II) stoichiometry to optimize the utilization of fluorescent and mercury light. Despite the adaptation of the photosynthetic apparatus to a small fraction of UV, long‐term exposure to mercury light did show a constant sensitivity of the photosynthetic efficiency of P. tricornutum to the phototoxic acridine. It is concluded that the prime receptor of photoenhanced toxicity may be unrelated to the photosynthetic machinery.