A study on volatile organic compounds (VOCs) produced by tropical ascomycetous yeasts
A study on volatile organic compounds (VOCs) produced by tropical ascomycetous yeasts
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DOI：
10.1023/a:1026064527932
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Davoli, P
中科院分区：
文献类型：
--
作者：
Buzzini, P;Martini, A;Davoli, P
作为一个程序的一部分，旨在选择菌株，这可能是感兴趣的天然香料分子的来源，挥发性有机化合物（VOCs）的生产由98个子囊菌酵母菌株（代表40种属于12属）分离自热带环境进行了调查。通过顶空固相微萃取（SPME）和气相色谱-质谱（GC-MS）分析和鉴定的化合物产生的挥发物进行采样。发现产生的VOC是醇（戊醇和异戊醇）、醛（2-甲基-2-己烯醛和2-异丙基-5-甲基-2-己烯醛）和酯（异丁酸乙酯、乙酸异丁酯、乙酸异戊酯、乙酸2-甲基丁酯、异戊酸乙酯、丙酸异戊酯和乙酸苯甲酯）。VOC谱的差异用于将酵母菌株聚类为25种VOC表型。不同的频率的VOC表型在三个特定的栖息地相关的不同的环境条件，可能会影响特定的酵母菌的选择。从生物技术的角度来看，这项研究揭示了从热带环境中分离的子囊菌酵母作为食品和香料工业相关VOCs的一个有前途的来源的潜力。
As a part of a program aiming at the selection of strains which might be of interest as sources of natural flavouring molecules, the production of volatile organic compounds (VOCs) by 98 ascomycetous yeast strains (representative of 40 species belonging to 12 genera) isolated from tropical environments was investigated. Volatiles produced were sampled by means of headspace solid-phase microextraction (SPME) and the compounds were analysed and identified by gas chromatography-mass spectroscopy (GC-MS). The VOCs produced were found to be alcohols (amyl alcohol and isoamyl alcohol), aldehydes (2-methyl-2-hexenal and 2-isopropyl-5-methyl-2-hexenal) and esters (ethyl isobutyrate, isobutyl acetate, isoamyl acetate, 2-methylbutyl acetate, ethyl isovalerate, isoamyl propionate and phenylmethyl acetate). Differences in VOC profiles were used to cluster the yeast strains into 25 VOC phenotypes. The different frequency of VOC phenotypes in three specific habitats was correlated to the divergent environmental conditions, possibly affecting the selection of specific yeasts. From a biotechnological viewpoint, this study reveals the potentiality of ascomycetous yeasts isolated from tropical environments as a promising source of VOCs relevant in food and fragrance industry.