Identification of genes expressed during hair follicle induction
Identification of genes expressed during hair follicle induction
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毛囊诱导过程中表达基因的鉴定
DOI：
10.1111/j.1346-8138.2010.01050.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
K. Sekiguchi
中科院分区：
文献类型：
--
作者：
D. Kiyozumi;A. Osada;Nagisa Sugimoto;Charles N. Weber;Y. Ono;T. Imai;A. Okada;K. Sekiguchi
毛囊是皮肤附属物之一，通过相互的上皮-间质相互作用而发育。虽然对毛囊发育的机制进行了大量的研究，但整个毛囊发育的分子机制仍然不清楚。为了进一步了解毛囊发育的分子基础，有必要确定驱动毛发形态发生的基因。作为一个初步的方法，我们试图确定与小鼠毛囊发育相关的基因产物。通过基于聚合酶链反应（PCR）的cDNA减法筛选触须毛基板中上调的基因。分别通过定量逆转录PCR分析和整体原位杂交评价由此分离的基因的毛发发育相关诱导和时空表达。最后，我们确定了四个基因，其上调和时空表达在发育中的毛囊得到证实。成功鉴定新的毛发发育相关基因将为进一步在分子水平上表征毛囊发育提供信息。
The hair follicle is one of the skin appendages that develops through reciprocal epithelial‐mesenchymal interactions. Although a large number of studies have been made on the mechanisms of hair follicle development, the whole molecular mechanism that governs hair follicle development remains poorly defined. To further understand the molecular basis of hair follicle development, it is necessary to identify genes that drive hair morphogenesis. As an initial approach, we attempted to identify gene products associated with mouse hair follicle development. Genes upregulated in the vibrissal hair placodes were screened by polymerase chain reaction (PCR)‐based cDNA subtraction. The genes thus isolated were evaluated for their hair development‐associated induction and spatiotemporal expression by quantitative reverse‐transcription‐PCR analysis and whole‐mount in situ hybridization, respectively. Finally, we identified four genes whose upregulation and spatiotemporal expression in developing hair follicles were confirmed. Successful identification of novel hair development‐associated genes will be informative as clues for further characterization of hair follicle development at the molecular level.
影响因子：
3.3
作者：
Yauch, RL;Berditchevski, F;Hemler, ME
通讯作者：
Hemler, ME
DOI：
10.1073/pnas.91.3.1133
发表时间：
1994-02
影响因子：
11.1
作者：
J. Wysolmerski;A. Broadus;Jing Zhou;E. Fuchs;L. Milstone;W. Philbrick
通讯作者：
J. Wysolmerski;A. Broadus;Jing Zhou;E. Fuchs;L. Milstone;W. Philbrick
影响因子：
10.5
作者：
Lowry, WE;Blanpain, C;Fuchs, E
通讯作者：
Fuchs, E