Positive correlation between single or combined genotypes of CYP1A1 and GSTM1 in relation to prostate cancer in Chilean people
Positive correlation between single or combined genotypes of CYP1A1 and GSTM1 in relation to prostate cancer in Chilean people
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DOI：
10.1002/pros.10274
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发表时间：
2003-08-22
期刊：
影响因子：
2.8
通讯作者：
Sepúlveda, LQ
中科院分区：
文献类型：
--
作者：
Acevedo, C;Opazo, JL;Sepúlveda, LQ
背景资料。前列腺癌是一种进展缓慢的疾病，在确诊前几十年就开始了。有研究表明，生物转化酶基因的遗传多态性可能会导致易感性的差异。在本研究中，在病例对照研究中，分析了细胞色素P1A1(Msp1)、谷胱甘肽转移酶M1(-/-)基因多态性与前列腺癌的关系。用聚合酶链式反应-限制性片段长度多态性方法同时检测Msp1和GSTM1(-/-)基因多态性。结果。在癌症患者中，M2变异等位基因频率(0.377)和GSTm1(-/-)频率(0.362)与对照组(分别为0.262和0.227)相比差异有统计学意义(P<0.05)。与仅携带CYP1A1或GSTM1的个体相比，联合携带CYP1A1和GSTM1罕见基因的个体的估计相对危险度(OR)更高。多因素Logistic回归分析显示，携带m2m2基因(OR=3.99；95%CI，1.27-12.54)、GST(-/-)基因(OR=2.75；95%CI，1.31-5.79)和m2m2/Gst(-)基因(OR=16.63；95%CI，1.67-165.48)的个体患前列腺癌的风险更高。(C)2003年Wiley-Liss，Inc.
BACKGROUND. The prostate cancer is a slowly progressing disease that begins decades prior to diagnosis. It has been suggested that there might be differences in susceptibility due to genetic polymorphisms in biotransformation enzyme genes. In the present work, associations between CYP1A1(Msp1), GSTM1(-/-) polymorphisms, and prostate cancer were analyzed in a case-control study.METHODS. Genomic DNA was isolated from peripheral blood samples, collected on EDTA. PCR-RFLP was used to determine simultaneously Msp1 and GSTM1 (-/-) polymorphisms. RESULTS. In cancer patients, frequency of m2 variant allele (0.377) and GSTM1(-/-) (0.362) showed statistically significant increases compared to the control group (0.262 and 0.227, respectively). The estimate relative risks (OR) were higher for individuals carrying combined CYP1A1 and GSTM1 rare genotypes, in relation to individuals carrying CYP1A1 or GSTM1 alone. Multivariate logistic regression analysis including confounding factors (age, digital examination, and PSA antigen) showed even higher risk for individuals carrying m2m2 genotype (OR = 3.99; 95% CI, 1.27-12.54), GST(-/-) genotype (OR = 2.75; 95% CI, 1.31-5.79), and m2m2/GST(-) genotype (OR = 16.63; 95% CI, 1.67-165.48).CONCLUSIONS. Taken together, these findings suggest that Chilean people carrying single or combined GSTM1 and CYP1A1 polymorphisms are more susceptible to prostate cancer. (C) 2003 Wiley-Liss, Inc.