Nitric oxide synthase activity.
Nitric oxide synthase activity.
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一氧化氮合酶活性。
DOI：
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发表时间：
1996
影响因子：
3.2
通讯作者：
W. Frederiks
中科院分区：
文献类型：
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作者：
W. Frederiks
我怀着极大的兴趣阅读了Mehdizadeh等人（19951）在《组织化学和细胞化学杂志》上发表的关于“大鼠肝脏切片中一氧化氮合酶活性的测定”的论文。到目前为止，NOS的原位定位是用免疫组织化学技术来检测蛋白质，或者用原位杂交技术来证明“A。NADPH黄递酶法被认为可证明NOS活性，但该方法不适用于在所有条件下检测酶活性（Beesley，1995）。因此，这是非常有用的，发展原位技术，显示NOS活性。Mehdizadeh等人（1995 a）提出的原理是基于一氧化氮刺激鸟苷酸环化酶活性的能力。这是一个原始的想法，并可能会产生一个有效的程序来检测NOS活性时，两种酶都存在于同一个细胞中，当一个有效的方法可用于证明可溶性鸟苷酸环化酶的活性。Mehdizadeh等人（1995年b）满足了最后一个先决条件。然而，关于两种相关酶的定位，Mehdizadeh等人（1995 a）假设NOS活性存在于枯否细胞中，可溶性鸟苷酸存在于肝细胞中。因此，一氧化氮从枯否细胞扩散到肝细胞。根据Mehdizadeh等人（1995年a）。通过将孵育介质中稳定剂的浓度从35%降低到30%，可以促进这种扩散。然而，这意味着所提出的组织化学方法不提供任何关于NOS活性的精确原位定位的信息，这是组织化学的特征。如果一氧化氮的扩散不受限制，则该方法可给出大鼠肝脏中总NOS活性的指示。然而，为了该目的，使用组织或细胞匀浆、细胞级分和亚细胞级分的成熟的生物化学方法是可用的（Curran等人，1989年）。第44卷，第11期，第1341页。1996年在美国出版。
With great interest I have read the paper of Mehdizadeh et al. (19951) in the Journal of Histochemirtry and Cytochemishy on “Measurement of nitric oxide synthase activity in sections of rat liver.’’ Until now, in situ localization of NOS was performed with immunohistochemical techniques, which detect the protein, or in situ hybridization, which demonstrates the “A. The NADPH diaphorase method has been claimed to demonstrate NOS activity, but this procedure is not appropriate to detect the enzyme activity under all conditions (Beesley, 1995). Therefore, it is very useful to develop in situ techniques for the demonstration of NOS activity. The principle introduced by Mehdizadeh et al. (1995a) is based on the capacity of nitric oxide to stimulate guanylate cyclase activity. This is an original idea, and may give rise to a valid procedure to detect NOS activity when both enzymes are present in the same cell and when a valid method is available to demonstrate soluble guanylate cyclase activity. The last prerequisite has been fulfilled by Mehdizadeh et al. (1995b). However, with respect to the localization of the two related enzymes, Mehdizadeh et al. (1995a) assume that NOS activity is present in Kupffer cells and that soluble guanylate is present in hepatocytes. As a consequence, diffusion of nitric oxide from Kupffer cells to hepatocytes should occur. According to Mehdizadeh et al. (1995a). this diffusion can be facilitated by decreasing the concentration of the stabilizer in the incubation medium from 35% to 30%. However, this means that the proposed histochemical approach does not provide any information on the precise in situ localization of NOS activity, which is the characteristic of histochemistry. The method may give indications for the total NOS activity in rat liver if diffusion of nitric oxide is not limited. However, for that purpose well-established biochemical methods are available using tissue or cell homogenates, cell fractions, and subcellular fractions (Curran et al., 1989). Vol. 44, No. 11, p. 1341. 1996 Printd in USA.