The chromaffin granule - plasma membrane interaction as a model for exocytosis: quantitative release of the soluble granular content.
The chromaffin granule - plasma membrane interaction as a model for exocytosis: quantitative release of the soluble granular content.
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嗜铬颗粒-质膜相互作用作为胞吐作用的模型：可溶性颗粒内容物的定量释放。
DOI：
10.1016/0006-291x(82)91967-2
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发表时间：
1982
影响因子：
3.1
通讯作者：
W. De Potter
中科院分区：
文献类型：
--
作者：
F. Konings;W. De Potter
牛肾上腺髓质膜以钙离子依赖的方式诱导嗜铬颗粒中的可溶性成分释放。这种相互作用可以通过改变温度来调节。在不同温度孵育的样品中，4种释放的可溶性标志物(儿茶酚胺、可溶性蛋白、三磷酸腺苷和多巴胺-β-羟基酶)浓度的相关性表明，这些标志物是同时释放的。它们的比例与嗜铬芬颗粒裂解物中测得的比例没有显著差异。这些结果表明，当与质膜孵育时，总颗粒内容物被释放。对游离儿茶酚胺的进一步分析表明，肾上腺素的优先释放。
Bovine adrenal medullary plasma membranes induce the release of soluble components from chromaffin granules in a Ca2+dependent manner. This interaction can be modulated by changing the temperature. Correlation of the concentrations of four released soluble markers (catecholamines, soluble protein, ATP and dopamine-β-hydroxylase) in samples incubated at different temperatures revealed that those markers were liberated simultaneously. Their ratio did not differ significantly from the ratio measured in chromaffin granule lysates. These results suggest the release of the total granular content upon incubation with plasma membranes. Further analysis of the free catecholamines showed a preferential release of adrenalin.