Quantifying the dynamic fluorescence quenching of phenanthrene and ofloxacin by dissolved humic acids
Quantifying the dynamic fluorescence quenching of phenanthrene and ofloxacin by dissolved humic acids
复制标题
溶解腐殖酸对菲和氧氟沙星的动态荧光猝灭进行定量
DOI：
10.1016/j.envpol.2014.10.029
复制
发表时间：
2015-01-01
影响因子：
8.9
通讯作者：
Pan, Bo
中科院分区：
文献类型：
--
作者：
Wang, Lin;Liang, Ni;Pan, Bo
荧光猝灭包括动态猝灭和静态猝灭，并且这两个过程都可以改变荧光剂的行为和反应性。然而，动态淬火很少量化。本研究结合透析平衡法和荧光猝灭法，比较动态和静态猝灭的贡献。结果表明，菲（PHE）与DHA的结合随着DHA疏水性的增加而增加，而氧氟沙星（OFQ）与DHA的相互作用则表现出相反的效果。对于PHE，动态猝灭对整体荧光猝灭的贡献在50%至82%的范围内，并且随着DHA疏水性的增加而降低至11%至58%。然而，OFL的动态猝灭从2%（类似于27%）增加到31%（类似于61%），其中DHA具有疏水性。结合模型化合物的结果，我们得出结论：DHA中的羧基可能是PHE动态猝灭的主要成分，并可能是OFL动态和静态猝灭的主要原因。需要广泛的研究来探索动态猝灭和化学/DHA性质的定量关系。(C)2014爱思唯尔有限公司版权所有。
Fluorescence quenching includes dynamic and static quenching, and both processes can alter the behavior and reactivity of the fluorescer. However, dynamic quenching is seldom quantified. This study combined dialysis equilibrium and fluorescence quenching methods to compare the contribution of dynamic and static quenching. The results indicate that phenanthrene (PHE)-DHA binding increased with DHA hydrophobicity, while ofloxacin (OFQ-DHA interaction showed the opposite effect. For PHE, the contribution of dynamic quenching to the overall fluorescence quenching was in the range of 50%similar to 82% and decreased to 11%similar to 58% with increased DHA hydrophobicity. However, OFL dynamic quenching increased from 2%similar to 27% to 31%similar to 61% with DHA hydrophobicity. Combining the results using model chemicals, we concluded that the carboxyl groups in DHA might be the primary components for PHE dynamic quenching and might be responsible for both dynamic and static quenching of OFL. Extensive study is needed to explore the quantitative relationship of dynamic quenching and chemical/DHA properties. (C) 2014 Elsevier Ltd. All rights reserved.