Effects of long-term treatment with bovine somatotropin on follicular dynamics and subsequent oocyte and blastocyst yield in an OPU-IVF program
Effects of long-term treatment with bovine somatotropin on follicular dynamics and subsequent oocyte and blastocyst yield in an OPU-IVF program
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DOI：
10.1016/s0093-691x(98)00047-8
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发表时间：
1998-04-01
期刊：
影响因子：
2.8
通讯作者：
de Kruif, A
中科院分区：
文献类型：
--
作者：
Bols, PEJ;Ysebaert, MT;de Kruif, A
14头15 - 22月龄、生殖道正常的荷斯坦-弗里舍小母牛被用于研究经阴道取卵（OPU）前长期rbst治疗对卵泡数量的影响。每隔11天两次注射氯前列醇2ml，同步动物的发情周期。选取平均体重相同的母牛分为两组（n=7），其中一组每周一皮下注射重组牛生长激素640 mg （rBST治疗组），另一组皮下注射生理盐水10 ml。两组小母牛每周两次在周一和周四使用5 Mhz换位器和一次性55 mm长20 g短斜针，真空压力约为13 ml水/min。实验持续10周（4月至6月），每只动物共接受10次注射，并向OPU提交20次。卵母细胞随后成熟并按照常规试管婴儿程序在每头奶牛的单独滴液中培养。每次OPU后立即取血肝素，测定血黄体酮浓度，以评估治疗和OPU程序对奶牛发情周期的影响。虽然结果显示rbst治疗组的卵泡总数和中等大小卵泡数量明显增加，但rbst治疗组和未治疗组的卵母细胞数量没有统计学差异。每头奶牛每次提取的平均卵母细胞数是相似的，实验组为6.4个，对照组为6.0个。此外，每头奶牛每次产犊的平均囊胚数在两组之间没有显著差异，rbst处理组为1.41个，对照组为1.53个。rbst处理组培养的卵母细胞发育到囊胚期的数量为22%，与对照组的25%无显著差异。重复的OPU似乎在治疗和未治疗的动物中诱导一定程度的不周期性。(C) 1998年Elsevier Science Inc。
Fourteen Holstein-Friesian heifers between 15 and 22 months of age with normal reproductive tracts were used in an experimental set up to investigate the effect of a long term rBST-treatment on the follicular population prior to transvaginal ovum pick-up (OPU). The estrous cycle of the animals was synchronized by means of a double injection of 2 ml cloprostenol 11 days apart. The heifers were divided in 2 equal groups (n=7) in which the animals had the same average body weight, one group receiving a weekly subcutaneous injection of 640 mg recombinantly derived bovine somatotropin (rBST) on Mondays (rBST-treatment group) and a control group, being injected with 10 ml of saline. Heifers in both groups were submitted to OPU twice a week on Mondays and Thursdays using a 5 Mhz transducer and a disposable, 55 mm long 20-g short bevelled needle at a vacuum pressure corresponding to approximately 13 ml water/min The experimental period lasted for 10 weeks (April to June), each animal receiving a total of 10 injections and being submitted to OPU for 20 times. Oocytes were subsequently matured and cultured in a separate drop per cow following conventional IVF procedures. A blood sample was taken on heparin immediately after each OPU session, for determination of blood progesterone concentrations to assess the influence of treatment and OPU procedure on the cow's estrous cycle. Although results show a significant increase in the total number of follicles and medium sized follicles in the rBST-treated group, no statistically significant different number of retrieved oocytes between the rBST-treated and nontreated group could be detected. The average number of retrieved oocytes per session per cow was comparable, being 6.4 for the treated and 6.0 for the control group. Additionally, the average number of blastocysts per cow per session did not differ significantly between groups, being 1.41 in the rBST-treated group and 1.53 in the control group. The number of cultured oocytes which developed to the blastocyst stage was 22% in the rBST-treated group, which was not significantly different from 25% in the control group. Repeated OPU appeared to induce a certain degree of acyclicity in both treated and nontreated animals. (C) 1998 by Elsevier Science Inc.