DNA: An extensible molecule
DNA: An extensible molecule
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DOI：
10.1126/science.271.5250.792
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发表时间：
1996-02-09
期刊：
影响因子：
56.9
通讯作者：
Caron, F
中科院分区：
文献类型：
--
作者：
Cluzel, P;Lebrun, A;Caron, F
测量了单个双链DNA分子的力-位移响应。引力在约70皮牛顿的平台上饱和，当DNA被拉伸约1.7倍其等高线长度时，该平台就会结束。这一行为揭示了一种高度合作的状态，在这里被称为S-DNA。添加插层剂可以抑制这种转变。该过程的分子模拟也产生了力平台，并为扩展形式提出了一种结构。这些结果可能会揭示涉及DNA延伸的生物过程，并为在以前未被探索的范围内对单个分子进行机械研究开辟道路。
The force-displacement response of a single duplex DNA molecule was measured. The force saturates at a plateau around 70 piconewtons, which ends when the DNA has been stretched about 1.7 times its contour length. This behavior reveals a highly cooperative transition to a state here termed S-DNA. Addition of an intercalator suppresses this transition. Molecular modeling of the process also yields a force plateau and suggests a structure for the extended form. These results may shed light on biological processes involving DNA extension and open the route for mechanical studies on individual molecules in a previously unexplored range.