Acid-insoluble human dentin as carrier material for recombinant human BMP-2
Acid-insoluble human dentin as carrier material for recombinant human BMP-2
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DOI：
10.1002/jbm.a.33236
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发表时间：
2012-03-01
影响因子：
4.9
通讯作者：
Arisue, Makoto
中科院分区：
文献类型：
--
作者：
Murata, Masaru;Sato, Daisuke;Arisue, Makoto
本研究的目的是评估人脱矿牙本质基质（DDM）与重组人骨形态发生蛋白-2（BMP-2）的骨诱导能力的增加。将人牙齿压碎，在0.6M HCl中完全脱矿，并冷冻干燥。牙齿衍生的材料被称为DDM。DDM呈颗粒状，大小为0.4 ~ 0.8mm。大鼠皮下组织BMP-2剂量依赖性研究表明，诱导骨和骨髓体积呈剂量依赖性增加。通过BMP-2（5.0 μ g）/DDM（70 mg）的骨诱导的时间过程研究进行了组织学和生物化学评估。组织学结果显示，BMP-2/DDM在DDM颗粒之间依次增加骨和骨髓。BMP-2/DDP诱导的组织中的钙含量与组织学结果一致。BMP-2/DDM中ALP活性在1周时达到最大值，随后逐渐下降。形态计量学分析显示，BMP-2/DDM在8、32周时占骨体积和骨髓体积的66.9%、79.0%，DDM占32.4%、21.0%。我们证实，BMP-2显着加速骨形成的酸不溶性人牙本质载体。这些结果表明，人DDM应该是一个有效的载体，提供BMP-2和上级支架成骨细胞。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part A，2012。
The aim of this study is to estimate the increase of bone-inductive potency by human demineralized dentin matrix (DDM) with recombinant human bone morphogenetic protein-2 (BMP-2). Human teeth were crushed, completely demineralized in 0.6M HCl, and freeze-dried. The tooth-derived material is called DDM. The shape of DDM was a particle type and its size varied from 0.4 to 0.8 mm. The BMP-2 dose-dependent study in the rat subcutaneous tissues demonstrated that the volume of induced bone and marrow increased at a dose-dependent manner. The time-course study of bone induction by the BMP-2 (5.0 mu g)/DDM (70 mg) was estimated histologically and biochemically. Histological findings showed that the BMP-2/DDM increased bone and marrow sequentially between the DDM particles. Calcium content in the BMP-2/DDM-induced tissue was compatible to the histological findings. ALP activity in the BMP-2/DDM showed a maximal value at 1 week and gradually decreased. The morphometric analysis demonstrated that the BMP-2/DDM showed 66.9%, 79.0% in the volume of bone and marrow, and 32.4%, 21.0% in that of DDM at 8, 32 weeks, respectively. We confirmed that BMP-2 significantly accelerated bone formation in the acid-insoluble human-dentin carriers. These results indicate that human DDM should be an effective carrier for delivering BMP-2 and superior scaffold for bone-forming cells. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.