DETERMINATION OF MALE-SPECIFIC GENE-EXPRESSION IN DROSOPHILA ACCESSORY-GLANDS
DETERMINATION OF MALE-SPECIFIC GENE-EXPRESSION IN DROSOPHILA ACCESSORY-GLANDS
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DOI：
10.1016/0012-1606(88)90253-9
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发表时间：
1988-03-01
影响因子：
2.7
通讯作者：
WOLFNER, MF
中科院分区：
文献类型：
--
作者：
CHAPMAN, KB;WOLFNER, MF
研究了三种雄性特异性体细胞转录本的发育调控。这些RNA仅在成年雄性附腺中合成，附腺是来自黑腹果蝇生殖盘的内部组织。这些雄性特异性转录物（msts）的表达受性别决定调控层次的控制，如在doublemex（dsx）、间性（ix）或Transformer-2（tra2）基因座处携带突变等位基因的染色体雌性中所有三种msts的表达所证明的。虽然转录的所有三个男性RNA的启动晚化蛹，温度变化的X/X; tra2ts2纯合子在发展过程中表明，这种表达是不可逆的确定较早，在第三龄幼虫。X/X的移位; TRA2TS 2纯合子与雄性决定温度仅在晚期幼虫期的持续时间内足以引起MSTS在成虫期的表达。这些成绩单的确定这一关键时期似乎与这些动物的附属腺的形态学测定的时间。因此，这些基因的表达可以通过它们在其中活跃的雄性特异性组织的形态学测定来指定。
The developmental regulation of three male-specific somatic transcripts is investigated. These RNAs are synthesized exclusively in the adult male accessory gland, an internal tissue derived from the genital disk of Drosophila melanogaster. The expression of these male-specific transcripts (msts) is under the cotrol of the sex determination regulatory hierarchy, as demonstrated by the expression of all three msts in chromosomal females carrying mutant alleles at the doublesex (dsx), intersex (ix), or transformer-2 (tra2) loci. Although transcription of all three male RNAs is initiated late in pupation, temperature shifts of X/X; tra2ts2 homozygotes during development indicated that this expression is irreversibly determined earlier, during the third larval instar. A shift of X/X; tra2ts2 homozygotes to the male-determining temperature only for the duration of the late larval period is sufficient to elicit the expression of the msts during the adult stage. This critical period for the determination of these transcripts appears to correlate with the time of morphological determination of the accessory gland in these animals. Thus, the expression of these genes could be specified by the morphological determination of the male-specific tissue in which they are active.