Study of the constitutively active form of the α subunit of rice heterotrimeric G proteins
Study of the constitutively active form of the α subunit of rice heterotrimeric G proteins
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DOI：
10.1093/pcp/pci036
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发表时间：
2005-02-01
影响因子：
4.9
通讯作者：
Iwasaki, Y
中科院分区：
文献类型：
--
作者：
Oki, K;Fujisawa, Y;Iwasaki, Y
我们使用定点突变工程师的两个组成型活性形式的α亚基的水稻异源三聚体G蛋白。从这些新的cDNA产生的重组蛋白具有GTP结合活性，但没有GTP酶活性。一种组成型活性α的嵌合基因。亚基被导入水稻突变体d1中，该突变体d1是a-亚基基因缺陷的。与正常栽培品种相比，所有的转化体基本上显示出野生型表型，尽管种子大小大幅增加，节间长度也显示出一些增加。
We used site-directed mutagenesis to engineer two constitutively active forms of the alpha subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the alpha. subunit was introduced into the rice mutant d1, which is defective for the a-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.