Modifications of the steroidogenic pathway during spontaneous and adrenocorticotropin-induced maturation of ovine fetal adrenal.
Modifications of the steroidogenic pathway during spontaneous and adrenocorticotropin-induced maturation of ovine fetal adrenal.
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绵羊胎儿肾上腺自发和促肾上腺皮质激素诱导成熟过程中类固醇生成途径的改变。
DOI：
10.1210/endo-110-2-500
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
J. Saez
中科院分区：
文献类型：
--
作者：
P. Durand;A. Cathiard;A. Locatelli;J. Saez
本文提出的研究旨在辨别在羊胎儿肾上腺发育过程中是否发生类固醇生成途径的改变。从 120 日龄胎儿（对照）、用 ACTH 灌注 5 天的 120 日龄胎儿（ACTH 处理）中分离出肾上腺细胞，并孵育新生羔羊。用 14C 标记的孕烯醇酮 [3β-羟基-5-孕烯醇-20-酮 (P5)]。 P5消失和标记黄体酮、11-脱氧皮质酮（21-羟基-4-孕烯-3,20-二酮）、皮质酮、17α-羟基孕烯醇酮（3β,17α-二羟基-5-孕烯-20-酮）、17α-二羟基孕酮的形成的时间进程（30、60、90和120分钟）测量了（17α-羟基-4-孕烯-3-酮）、11-脱氧皮质醇（17α,21-二羟基-4-孕烯-3,20-二酮）和皮质醇。对照胎儿中 P5 从培养基中消失的速度比 ACTH 处理的胎儿或新生羔羊慢得多。在对照胎儿中，2小时后，只有45%的P5消失，其中85%以上通过17-de代谢...
The studies presented herein were undertaken to discern if modifications of the steroidogenic pathway occur during ovine fetal adrenal development. Isolated adrenal cells from 120-day-old fetuses (controls), 120-day-old fetuses which were perfused for 5 days with ACTH (ACTH-treated), and newborn lambs were incubated. with 14C-labeled pregnenolone [3β-hydroxy-5-pregnen-20-one (P5)]. The time courses (30, 60, 90, and 120 min) of P5 disappearance and the formation of labeled progesterone, 11-deoxycorticosterone (21-hydroxy-4-pregnene-3,20-dione), corticosterone, 17α-hydroxypregnenolone (3β,17α-dihydroxy-5-pregnene-20-one), 17α-dihydroxyprogesterone (17α-hydroxy-4-pregnene-3-one), 11-deoxycortisol (17α,21-dihydroxy-4-pregnene-3,20-dione), and cortisol were measured. The rate of P5, disappearance from the medium was much slower in control fetuses than in ACTH-treated fetuses or newborn lambs. In control fetuses, after 2 h, only 45% of P5 had disappeared, of which more than 85% was metabolized through the 17-de...