Nucleus-translocated matrix metalloprotease 1 regulates innate immune response in Pacific abalone (Haliotis discus hannai)
Nucleus-translocated matrix metalloprotease 1 regulates innate immune response in Pacific abalone (Haliotis discus hannai)
复制标题
核转位基质金属蛋白酶 1 调节太平洋鲍鱼（Haliotis Discus hannai）的先天免疫反应
DOI：
10.1016/j.fsi.2018.10.017
复制
发表时间：
2019-01-01
影响因子：
4.7
通讯作者：
Cao, Min-Jie
中科院分区：
文献类型：
--
作者：
Chen, Yu-Lei;Li, Wan-Yu;Cao, Min-Jie
鲍鱼作为我国沿海地区重要的经济贝类，容易受到细菌感染，尤其是副溶血性弧菌（V parahemolyticus）。基质金属蛋白酶（MMPs）在哺乳动物的免疫应答中已被广泛研究。然而，MMP在鲍鱼天然免疫系统中的作用尚不清楚。本研究探讨了MMP-1在皱纹盘鲍免疫应答中的作用。结果表明，副溶血性弧菌感染后，MMP-1、同种异体移植物炎症因子1（AIF-1）、巨噬细胞表达基因1（MPEG-1）和TPA诱导序列11家族蛋白（Tis 11 FP）等免疫相关基因的表达均显著升高。值得注意的是，MMP-1的沉默降低了这些基因的表达，表明MMP-1是副溶血性弧菌感染的上游调控因子。进一步分析表明，MMP-1参与细胞免疫（吞噬、凋亡）和体液免疫[超氧化物歧化酶（SOD）、碱性磷酸酶（ALP）、酸性磷酸酶（ACP）]的调节。有趣的是，细胞外分布的MMP-1可以易位到血细胞的细胞核中，从而作为转录调节因子发挥作用，或者通过蛋白水解选择性地激活或灭活其他成分。因此，我们的研究确定了MMP-1在鲍鱼抗副溶血性弧菌感染的天然免疫中的重要作用，这是首次报道鲍鱼MMP的研究。
As an important economical shellfish in coastal area of China, abalone is susceptible to bacterial infection, especially Vibiro parahemolyticus (V parahemolyticus). Matrix metalloproteinases (MMPs) have been extensively investigated in the immune response of mammals. However, little is known about the involvement of MMP in abalone innate immune system against pathogen infection. In this study, the role of MMP-1 in the immune response of Pacific abalone (Haliotis discus hannai) was explored. The results showed that V. parahemolyticus infection induced significantly elevated expression of MMP-1 as well as immune related genes including allograft inflammatory factor 1 (AIF-1), macrophage expressed gene 1 (MPEG-1) and TPA-inducible sequence 11 family protein (Tis11FP). Notably, silencing of MMP-1 reduced the expression of these genes, suggesting that MMP-1 was an upstream regulatory factor in V. parahemolyticus infection. Further analysis showed that MMP-1 was engaged in the regulation of cellular (phagocytosis, apoptosis) and humoral [superoxide dismutase (SOD), alkaline phosphatase (ALP), acid phosphatase (ACP)] immunity. Interestingly, the extracellularly distributed MMP-1 could be translocated to the nuclei of hemocytes, thereby functioning as a transcriptional regulator or by selectively activating or inactivating other components through proteolysis. Hence, our study established an important role of MMP-1 in abalone innate immunity against V. parahemolyticus infection and it represented the first report on the investigation of MMP in abalone.