Uptake of dehydroepiandrosterone-3-sulfate by isolated trophoblasts from human term placenta, JEG-3, BeWo, Jar, BHK cells, and BHK cells transfected with human sterylsulfatase-cDNA
Uptake of dehydroepiandrosterone-3-sulfate by isolated trophoblasts from human term placenta, JEG-3, BeWo, Jar, BHK cells, and BHK cells transfected with human sterylsulfatase-cDNA
复制标题
人足月胎盘分离的滋养层细胞、JEG-3、BeWo、Jar、BHK 细胞和转染人甾醇硫酸酯酶-cDNA 的 BHK 细胞对 3-硫酸脱氢表雄酮的摄取
DOI：
10.1016/s0960-0760(99)00138-7
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Sabine Simon
中科院分区：
文献类型：
--
作者：
B. Ugele;Sabine Simon
人胎盘缺乏17α-羟化酶/17-20-裂解酶，因此无法将胆固醇转化为雌激素。因此，滋养层细胞的雌激素合成依赖于母体和胎儿血液中前体物质的供应，例如脱氢表雄酮-3-硫酸酯(DHEA-S)和16α-羟基-脱氢表雄酮-3-硫酸酯。为了研究这些阴离子亲水前体的细胞内化，研究了分离的细胞滋养层细胞、绒毛膜癌细胞系（JEG-3、BeWo、Jar）、用人甾醇硫酸酯酶-cDNA转染的BHK和BHK细胞（BHK-STS细胞）对[3H]-/[35S]-DHEA-S和[3H]-牛磺胆酸盐的摄取。此外，测量了悬浮液和相应细胞匀浆中这些细胞的甾醇硫酸酯酶活性。在与 [3H]-DHEA-S 或 [35S]-DHEA-S 孵育的前 5 分钟内，细胞滋养层细胞的放射性显着增加，而 JEG-3、Jar、BHK 和 BHK-STS 细胞的放射性没有增加。 BeWo细胞的放射性略有增加。对于所有细胞类型，两种底物的摄取没有显着差异。在与[3H]-牛磺胆酸盐一起孵育期间，细胞滋养层的放射性没有增加。细胞滋养层匀浆的甾醇硫酸酯酶活性显着低于细胞滋养层悬浮液。 BHK-STS、JEG-3或BeWo细胞匀浆的甾醇硫酸酯酶活性显着高于相应的细胞悬液。在 BHK 和 Jar 细胞中未检测到甾醇硫酸酯酶活性。细胞滋养层无需事先水解即可吸收 DHEA-S。 BHK、BHK-STS、JEG-3 和 Jar 细胞不吸收 DHEA-S，而 BeWo 细胞缓慢吸收 DHEA-S。在细胞滋养细胞中，细胞外 DHEA-S 快速获得细胞内的甾醇硫酸酯酶，而在绒毛膜癌和 BHK-STS 细胞中，DHEA-S 与甾醇硫酸酯酶的接近受到限制。我们的结果表明，细胞滋养层的摄取是由在绒毛膜癌或BHK细胞中不表达并且与已知的牛磺胆酸盐转运有机阴离子转运多肽不同的载体介导的。
The human placenta lacks the enzyme 17α-hydroxylase/17-20-lyase, and is thus unable to convert cholesterol into estrogens. Therefore estrogen synthesis of trophoblast cells depends on the supply of precursors such as dehydroepiandrosterone-3-sulfate (DHEA-S) and 16α-hydroxy-dehydroepiandrosterone-3-sulfate by maternal and fetal blood. To investigate the cellular internalisation of these anionic hydrophilic precursors, the uptake of [3H]-/[35S]-DHEA-S and [3H]-taurocholate by isolated cytotrophoblasts, cells of choriocarcinoma cell lines (JEG-3, BeWo, Jar), BHK and BHK cells transfected with human sterylsulfatase-cDNA (BHK-STS cells) was studied. Furthermore, the activity of sterylsulfatase of these cells in suspension and in corresponding cell homogenate was measured. During the first 5 min of incubation with [3H]-DHEA-S or [35S]-DHEA-S, radioactivity of cytotrophoblasts increased significantly, while radioactivity of JEG-3, Jar, BHK and BHK-STS cells did not increase. Radioactivity of BeWo cells increased slightly. For all cell types, there was no significant difference for uptake of either substrate. During incubation with [3H]-taurocholate, radioactivity of cytotrophoblasts did not increase. Sterylsulfatase activity of cytotrophoblast homogenate was significantly lower than that of cytotrophoblast suspension. Sterylsulfatase activity of BHK-STS, JEG-3 or BeWo cell homogenate was significantly higher than that of the corresponding cell suspension. In BHK and Jar cells sterylsulfatase activity was not detectable. Cytotrophoblasts take up DHEA-S without prior hydrolysis. BHK, BHK-STS, JEG-3, and Jar cells do not take up and BeWo cells slowly take up DHEA-S. In cytotrophoblasts extracellular DHEA-S rapidly gains access to intracellular sterylsulfatase, while in choriocarcinoma and BHK-STS cells access of DHEA-S to sterylsulfatase is limited. Our results indicate, that uptake by cytotrophoblasts is mediated by a carrier which is not expressed in choriocarcinoma or BHK cells and which is different from the known taurocholate-transporting organic anion transporting polypetides.
登录
查看更多内容
人胎盘刷状缘膜囊泡中的硫胺素转运。
DOI：
10.1016/s0005-2736(98)00019-4
发表时间：
1998
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Grassl,SM
通讯作者：
Grassl,SM
DOI：
10.1073/pnas.91.1.133
发表时间：
1994-01-04
影响因子：
11.1
作者：
JACQUEMIN, E;HAGENBUCH, B;MEIER, PJ
通讯作者：
MEIER, PJ
影响因子：
--
作者：
VANDYKE, RW;STEPHENS, JE;SCHARSCHMIDT, BF
通讯作者：
SCHARSCHMIDT, BF
影响因子：
4.8
作者：
KLIMAN, HJ;NESTLER, JE;STRAUSS, JF
通讯作者：
STRAUSS, JF
影响因子：
29.4
作者：
KULLAKUBLICK, GA;HAGENBUCH, B;MEIER, PJ
通讯作者：
MEIER, PJ