Effect of smoking on gingival crevicular fluid cytokine profile during experimental gingivitis
Effect of smoking on gingival crevicular fluid cytokine profile during experimental gingivitis
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DOI：
10.1034/j.1600-051x.2003.00416.x
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发表时间：
2003-11-01
影响因子：
6.7
通讯作者：
Mombelli, A
中科院分区：
文献类型：
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作者：
Giannopoulou, C;Cappuyns, I;Mombelli, A
背景：吸烟是牙周病发病的重要危险因素，可影响龈下微生物区系和宿主反应。目的：本研究旨在探讨吸烟对人实验性牙周炎过程中龈沟液中白介素1β(IL-1β)、白介素4(IL-4)和白介素8(IL-8)的影响。经过专业的清洁后，他们进行了最佳的卫生处理，以达到完美的临床牙龈健康。口腔卫生措施停止10天。临床指标包括菌斑指数(PI)、牙龈指数(GI)、探诊袋深度(PPD)和探诊出血量(BOP)，分别于实验前2天(第2天)、开始时(第0天)和实验结束时(第10天)进行评估。同时，用硬膜孔滤膜从每个患者的12个部位收集牙周液。结果：临床资料显示吸烟者和非吸烟者在实验过程中PI、GI和BOP评分均有增加。尽管在第10天的PI方面没有发现差异，吸烟者的GI和BOP明显低于非吸烟者(p
Background: Cigarette smoking is a significant risk factor in the pathogenesis of periodontal disease, able to influence both the subgingival microbiota and host responses.Aim: The aim of the present study was to determine the influence of smoking on the amount of IL-1beta, IL-4 and IL-8 in gingival crevicular fluid (GCF) during experimental gingivitis in humans.Material and Methods: Twenty-two healthy subjects, 10 smokers and 12 non-smokers, participated in the study. After professional cleaning, they performed optimal hygiene to reach perfect clinical gingival health. Oral hygiene measures were ceased for a period of 10 days. Clinical indices, including plaque index (PI), gingival index (GI), probing pocket depth (PPD) and bleeding on probing (BOP), were assessed 2 days before (day -2), at the beginning (day 0) and at the end of the experimental gingivitis period (day 10). At the same time, GCF was collected from 12 sites in each patient, by means of durapore filter membranes. Total amounts of IL-1beta, IL-4 and IL-8 were determined by enzyme-linked immunoadsorbent assay.Results: Clinical data revealed that both smokers and non-smokers showed an increase in PI, GI and BOP scores during the experiment. Although no differences were noted with regard to PI at day 10, the GI and BOP were significantly less pronounced in smokers than non-smokers (p