Evaluation of basal media for micropropagation of four highbush blueberry cultivars
Evaluation of basal media for micropropagation of four highbush blueberry cultivars
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DOI：
10.1016/j.scienta.2008.06.028
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发表时间：
2008-12
影响因子：
4.3
通讯作者：
T. Tetsumura;Yasuyo Matsumoto;Makiko Sato;C. Honsho;K. Yamashita;H. Komatsu;Y. Sugimoto;H. Kunitake
中科院分区：
文献类型：
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作者：
T. Tetsumura;Yasuyo Matsumoto;Makiko Sato;C. Honsho;K. Yamashita;H. Komatsu;Y. Sugimoto;H. Kunitake
比较了 Murashige 和 Skoog 培养基 (MS)、木本植物培养基 (WPM) 以及等份 MS 和 WPM (MW) 的混合物对四种高丛蓝莓 (Vaccinium spp.) 品种的体外芽增殖和生根的影响。在繁殖阶段，WPM 上的芽表现出比其他芽更差的生长。 MW 产生了最好的芽生长。 MS 上的芽生长良好，但在繁殖阶段倾向于过度含水，尤其是“Bluecrop”芽。在 WPM 上倍增的芽中生根最差，而在 MS 上获得“Bluecrop”芽的最佳生根率，在 MW 上获得“O’Neal”芽的最佳生根率。生根的芽发育良好，上盆后很容易适应环境。
Murashige and Skoog medium (MS), woody plant medium (WPM), and a mixture of equal parts of MS and WPM (MW) were compared for in vitro shoot proliferation and rooting of four highbush blueberry (Vaccinium spp.) cultivars. During the multiplication stage, the shoots on WPM showed worse growth than the other shoots. The MW produced the best shoot growth. The shoots on MS grew well but tended toward hyperhydricity in the multiplication stage, especially in the case of ‘Bluecrop’ shoots. Rooting was worst in the shoots multiplied on WPM, while the best rooting percentage of ‘Bluecrop’ shoots was obtained on MS, and that of ‘O’Neal’ on MW. The rooted shoots developed a good root system and were easily acclimatized after potting.