Nucleotide sequence of the uhp region of Escherichia coli
Nucleotide sequence of the uhp region of Escherichia coli
复制标题
大肠杆菌uhp区的核苷酸序列
作者：
M. Friedrich;R. Kadner
大肠杆菌uhp区域编码介导许多糖磷酸的摄取的转运系统以及负责通过外部葡萄糖6-磷酸诱导该转运系统的调节组分。通过分析携带亚克隆区或转座子插入的质粒的互补行为和多肽编码能力，已经鉴定出四个uhp基因。测定含有ilvBN操纵子的3'末端和整个uhp区域的6.5-内切酶区段的核苷酸序列。四个开放阅读框被确定在不同的uhp基因预期的位置，所有的方向相同，逆时针方向相对于遗传图谱。从核苷酸序列预测的多肽的性质与它们观察到的特征是一致的。196个氨基酸的UhpA多肽具有可溶性蛋白质的组成特征，并与许多调节激活剂和抑制剂的DNA结合区具有同源性。518个氨基酸的UhpB和199个氨基酸的UhpC调节蛋白含有大量的疏水性片段。类似地，463个氨基酸的UhpT转运蛋白是具有许多潜在跨膜区段的疏水蛋白。UhpC调节蛋白与UhpT的一部分具有实质性的序列同源性，这表明该调节蛋白可能是通过转运蛋白基因的重复而进化的，并且其在跨膜信号传导中的作用可能涉及糖-磷酸结合位点和类似于转运蛋白的跨膜取向。
The Escherichia coli uhp region encodes the transport system that mediates the uptake of a number of sugar phosphates as well as the regulatory components that are responsible for induction of this transport system by external glucose 6-phosphate. Four uhp genes have been identified by analysis of the complementation behavior and polypeptide coding capacity of plasmids carrying subcloned regions or transposon insertions. The nucleotide sequence of a 6.5-kilobase segment that contains the 3' end of the ilvBN operon and the entire uhp region was determined. Four open reading frames were identified in the locations expected for the various uhp genes; all were oriented in the same direction, counterclockwise relative to the genetic map. The properties of the polypeptides predicted from the nucleotide sequence were consistent with their observed features. The 196-amino-acid UhpA polypeptide has the composition characteristic of a soluble protein and bears homology to the DNA-binding regions of many regulatory activators and repressors. The 518-amino-acid UhpB and the 199-amino-acid UhpC regulatory proteins contain substantial segments of hydrophobic character. Similarly, the 463-amino-acid UhpT transporter is a hydrophobic protein with numerous potential transmembrane segments. The UhpC regulatory protein has substantial sequence homology to part of UhpT, suggesting that this regulatory protein might have evolved by duplication of the gene for the transporter and that its role in transmembrane signaling may involve sugar-phosphate-binding sites and transmembrane orientations similar to those of the transport protein.
大肠杆菌 K-12 的 ilvB 基因座是编码乙酰羟酸合酶 I 两个亚基的操纵子。
影响因子：
14.9
作者：
Friden,P;Donegan,J;Mullen,J;Tsui,P;Freundlich,M;Eoyang,L;Weber,R;Silverman,PM
通讯作者：
Silverman,PM
影响因子：
14.9
作者：
SHARP, PM;LI, WH
通讯作者：
LI, WH