Effects of proteoglycan extracted from nasal cartilage of salmon heads on maturation of dendritic cells derived from human peripheral blood monocytes.
Effects of proteoglycan extracted from nasal cartilage of salmon heads on maturation of dendritic cells derived from human peripheral blood monocytes.
复制标题
从鲑鱼头鼻软骨中提取的蛋白多糖对人外周血单核细胞来源的树突状细胞成熟的影响。
DOI：
10.1248/bpb.33.311
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发表时间：
2010
影响因子：
2
通讯作者：
I. Kashiwakura
中科院分区：
文献类型：
--
作者：
H. Yoshino;Kenji Takahashi;S. Monzen;I. Kashiwakura
树突状细胞(DC)在免疫系统中发挥着重要作用。从未成熟DC(IDCs)向成熟DC(MDCs)的转变需要适当的刺激，如促炎细胞因子。蛋白多糖(PG)是细胞外基质的主要成分之一，已知某些类型的PG可诱导小鼠DC成熟。最近的研究调查了从鲑鱼头鼻软骨中提取的PG(S-PG)的潜在益处。本研究探讨S-PG对人单核细胞来源的DC成熟的影响。用适当的细胞因子从人单核细胞中制备DC，然后用S-PG单独刺激。在另一项实验中，用促炎细胞因子(Mix)和S-PG联合刺激IDCs。虽然S-PG单独刺激不能诱导DC表型成熟，但S-PG单独刺激的DC表面CD40的表达低于DC。相反，MIX+S-PG刺激的DC的表型和功能特征与MIX单独刺激的DC相似。因此，S-PG对人单核细胞来源的DC的成熟没有明显的影响。
Dendritic cells (DCs) play an essential role in the immune system. The transition from immature DC (iDCs) to mature DCs (mDCs) requires appropriate stimuli such as pro-inflammatory cytokines. Proteoglycans (PGs) are one of the main components of extracellular matrix, and some types of PGs are known to induce maturation of murine DCs. Recent studies have investigated the potential benefits of PG from nasal cartilage of salmon head (S-PG). This study investigated the effects of S-PG on maturation of human monocyte-derived DCs. iDCs were prepared from human monocytes using the appropriate cytokines and then stimulated by S-PG alone. In another experiment, iDCs were stimulated by a combination of pro-inflammatory cytokines (MIX) plus S-PG. Although the stimulation of S-PG alone did not induce phenotypic maturation from iDCs, CD40 expression on DCs stimulated by S-PG alone was lower than that of iDCs. In contrast, the phenotypic and functional characteristics of DCs stimulated by MIX+S-PG were similar to those of DCs stimulated by MIX alone. As a result, S-PG did not demonstrate a significant effect with regard to maturation of human monocyte-derived DCs.