The role of oxidation and enzymatic hydrolysis on the in vivo degradation of trimethylene carbonate based photocrosslinkable elastomers
The role of oxidation and enzymatic hydrolysis on the in vivo degradation of trimethylene carbonate based photocrosslinkable elastomers
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DOI：
10.1016/j.biomaterials.2008.09.038
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Amsden, Brian G.
中科院分区：
文献类型：
--
作者：
Chapanian, Rafi;Tse, M. Yat;Amsden, Brian G.
研究了含三亚甲基碳酸酯（TMC）弹性体在体内的降解情况，并通过体外酶降解和氧化降解探讨了TMC弹性体的降解机理。弹性体通过TMC和等摩尔量的TMC和ε-己内酯（CL）的预聚物的UV引发交联来制备。降解过程之后，通过衰减全反射红外（ATR-FTIR）光谱研究机械性能，质量损失，吸水率，溶胶含量，差示扫描量热法和表面化学的变化。在体内降解过程中，TMC和TMCCL弹性体表现出表面侵蚀。TMC弹性体的组织反应强度更大。两种弹性体在体外胆固醇酯酶溶液中均表现出降解，但体内降解速率与体外降解速率之间无平行关系。只有TMCCL弹性体在脂肪酶中降解。在稳定的超氧阴离子在体外介质中的降解与所观察到的体内降解结果一致，表明通过粘附吞噬细胞在这些弹性体的降解中分泌这种活性氧物质的氧化起主导作用。(C)2008爱思唯尔有限公司保留所有权利。
The in vivo degradation of trimethylene carbonate (TMC) containing elastomers was investigated, and the mechanism of degradation explored through in vitro degradation under enzymatic and oxidative conditions. The elastomers were prepared via UV initiated crosslinking of prepolymers of TMC and equimolar amounts of TMC and epsilon-caprolactone (CL). The degradation process was followed by investigating the changes in the mechanical properties, mass loss, water uptake, sol content, differential scanning calorimetry, and Surface chemistry through attenuated total reflectance infrared (ATR-FTIR) spectroscopy. During in vivo degradation, TMC and TMCCL elastomers exhibited surface erosion. The tissue response was of greater intensity in the case of the TMC elastomer. Both elastomers exhibited degradation in cholesterol esterase containing solutions in vitro, but no parallels were found between the rate of in vivo degradation and the rate of in vitro degradation. Only the TMCCL elastomer degraded in lipase. Degradation in a stable superoxide anion in vitro medium was consistent with the observed in vivo degradation results, indicating a dominant role of oxidation through the secretion of this reactive oxygen species by adherent phagocytic cells in the degradation of these elastomers. (C) 2008 Elsevier Ltd. All rights reserved.