Effects of triclosan on the early life stages and reproduction of medaka Oryzias latipes and induction of hepatic vitellogenin
Effects of triclosan on the early life stages and reproduction of medaka Oryzias latipes and induction of hepatic vitellogenin
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DOI：
10.1016/j.aquatox.2003.12.005
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发表时间：
2004-04-14
影响因子：
4.5
通讯作者：
Arizono, K
中科院分区：
文献类型：
--
作者：
Ishibashi, H;Matsumura, N;Arizono, K
三氯生（2，4，4 '-三氯-2'-羟基二苯醚）广泛用作各种工业产品的抗菌剂，如纺织品、肥皂、洗发水、液体牙膏和化妆品，经常在废水中检出。在这项研究中，TCS的影响，。对青鳉的早期生活史和繁殖进行了研究。TCS对24 h龄幼虫的96 h半数致死浓度值为602 mug/l。受精卵暴露于313 mug/l TCS 14天的孵化率和孵化时间分别显著降低和延迟。TCS 21天暴露期对配对青鳉生殖影响的评估显示，对照组和20、100和200 μ g/l TCS处理组之间的产卵数和生育力无显著差异。然而，在20和100 mug/l TCS处理的雄性中，肝卵黄蛋白原浓度显著增加。在F-1代中，尽管20 μ g/l处理的胚胎孵化显示出不利影响，但孵化率与TCS处理水平之间没有剂量-反应关系。这些结果表明，TCS对青鳉的早期生命阶段具有较高的毒性，TCS的代谢产物可能是一种弱雌激素化合物，具有诱导雄性青鳉卵黄蛋白原的潜力，但对生殖成功和后代没有不良影响。(C)2003 Elsevier B. V.保留所有权利。
Triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) is widely used as antibacterial agent in various industrial products, such as textile goods, soap; shampoo, liquid toothpaste and cosmetics, and often detected in wastewater effluent. In this study, the effects of TCS on the,. early life stages and reproduction of medaka (Oryzias latipes) were investigated. The 96-h median lethal concentration value of TCS for 24-h-old larvae was 602 mug/l. The hatchability and time to hatching in fertilized eggs exposed to 313 mug/l TCS for 14 days were significantly decreased and delayed, respectively. An assessment of the effects of a TCS 21-day exposure period on the reproduction of paired medaka showed no significant differences in the number of eggs produced and fertility among the control and 20, 100 and 200 mug/l TCS treatment groups. However, concentrations of hepatic vitellogenin were increased significantly in males treated with TCS at 20 and 100 mug/l. In the F-1 generations, although the hatching of embryos in the 20 mug/l treatment showed adverse effects, there was no dose-response relationship between hatchability and TCS treatment levels. These results suggest that TCS has high toxicity on the early life stages of medaka, and that the metabolite of TCS may be a weak estrogenic compound with the potential to induce vitellogenin in male medaka but with no adverse effect on reproductive success and offspring. (C) 2003 Elsevier B.V. All rights reserved.