EPIDERMAL CHALONE - PRELIMINARY ATTEMPT AT ISOLATION
EPIDERMAL CHALONE - PRELIMINARY ATTEMPT AT ISOLATION
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DOI：
10.1016/0014-4827(64)90172-7
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发表时间：
1964-01-01
影响因子：
3.7
通讯作者：
HEWETT, CL
中科院分区：
文献类型：
--
作者：
BULLOUGH, WS;LAURENCE, EB;HEWETT, CL
表皮抑素是一种水溶性的非透析性物质，可被乙醇沉淀，并可被生物处理破坏，它与肾上腺素作为辅因子，能明显抑制成年小鼠表皮的有丝分裂率。它不能在水溶液中存活很长时间，即使在-20[degree]C.，但它被证明在冷冻干燥后至少能存活一年。已经进行了初步尝试，通过用乙醇分级沉淀来纯化水提取物。已经发现，当醇浓度从70%提高到80%时，超过80%的回收的查尔酮存在于所获得的沉淀物中。这些结果与表皮抑素可能被证明是一种蛋白质的可能性并不矛盾。
The epidermal chalone, which with adrenalin as cofactor evidently depresses the epidermal mitotic rate in an adult mouse, is shown to be water soluble, non-dialysable, precipitated by alcohol, and destroyed by bioling. It does not survive long in water solution, even at -20[degree]C., but it has been shown to survive for at least a year when lyophilized. Preliminary attempts have been made to purify the water extract by fractional precipitation with alcohol. It has been found that more than 80% of the chalone recovered was present in the precipitate obtained when the alcohol concentration was raised from 70 to 80%. These results are not inconsistent with the possibility that the epidermal chalone may prove to be a protein.