HUMAN-ANTIBODY RESPONSES TO EPITOPES ON THE PLASMODIUM-FALCIPARUM GAMETOCYTE ANTIGEN PFS 48/45 AND THEIR RELATIONSHIP TO INFECTIVITY OF GAMETOCYTE CARRIERS
HUMAN-ANTIBODY RESPONSES TO EPITOPES ON THE PLASMODIUM-FALCIPARUM GAMETOCYTE ANTIGEN PFS 48/45 AND THEIR RELATIONSHIP TO INFECTIVITY OF GAMETOCYTE CARRIERS
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DOI：
10.4269/ajtmh.1992.46.711
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发表时间：
1992-06-01
影响因子：
3.3
通讯作者：
BATTISTUTTA, D
中科院分区：
文献类型：
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作者：
GRAVES, PM;DOUBROVSKY, A;BATTISTUTTA, D
用竞争性酶联免疫吸附试验研究了人血清中识别恶性疟原虫配子体抗原Pfs 48/45表位I、IIa、III和IV的抗体。在巴布亚新几内亚马当的三个村庄中，超过三分之一的居民对表位I、IIa和III有反应，不同村庄或不同时间的差异很小。阳性血清按年龄呈双峰分布，5-9岁和> 20岁年龄组应答者比例最高。这些数据表明恶性疟原虫配子体携带者中抗表位IIa和III的抗体的流行率低于非携带者。增强单克隆抗体结合表位IIa和III也观察到更频繁的血清配子载体。来自巴布亚新几内亚和泰国的配子母细胞携带者的血清，其对蚊子的感染性已经被测试，用于检查识别特定表位与感染性之间的关系。恶性疟原虫配子体携带者缺乏感染性与其血清中抗体识别Pfs 48/45上的表位IIa之间存在显著关联。
Antibodies in human sera recognizing epitopes I, IIa, III, and IV on the Plasmodium falciparum gametocyte antigen Pfs 48/45 have been investigated by competitive enzyme-linked immunosorbent assay. More than one-third of the residents of three villages in Madang, Papua New Guinea responded to epitopes I, IIa and III, with little variation by village or with time. There was a bimodal distribution of positive sera by age, with the highest proportion of responders in the 5-9- and > 20-year-old age groups. The data suggest a lower prevalence of antibodies against epitopes IIa and III in P. falciparum gametocyte carriers than in non-carriers. Enhancement of binding of monoclonal antibodies to epitopes IIa and III was also observed more frequently with sera from gametocyte carriers. Sera from gametocyte carriers in Papua New Guinea and Thailand, whose infectivity to mosquitoes had been tested, were used to examine the relationship between recognition of particular epitopes and infectivity. There was a significant association between lack of infectivity of P. falciparum gametocyte carriers and recognition of epitope IIa on Pfs 48/45 by antibodies in their sera.