NMR in the SPINE structural proteomics project
NMR in the SPINE structural proteomics project
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DOI：
10.1107/s0907444906032070
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Kaptein, R.
中科院分区：
文献类型：
--
作者：
Ab, E.;Atkinson, A. R.;Kaptein, R.
本文介绍了在欧洲结构蛋白质组学（SPINE）财团的NMR光谱组的发展，作用和贡献。围绕蛋白质NMR结构测定的高通量（HTP）管道的开发，从样品制备、数据采集、数据处理、数据分析到结构测定的各个方面都在灵敏度、自动化、速度、鲁棒性和验证方面得到了改进。具体的亮点是无质子C-13-直接检测方法和推断结构测定（ISD）。除了技术改进之外，这些方法已被应用于提供超过60种蛋白质的NMR结构，其中有5种未能结晶。在结构蛋白质组学管道中包含NMR光谱法提高了蛋白质结构测定的成功率。
This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe ( SPINE) consortium. Focusing on the development of high- throughput ( HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless C-13-direct detection methods and inferential structure determinations ( ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.