Pituitary adenylate cyclase activating polypeptide innervation of the rat female reproductive tract and the associated paracervical ganglia: Effect of capsaicin
Pituitary adenylate cyclase activating polypeptide innervation of the rat female reproductive tract and the associated paracervical ganglia: Effect of capsaicin
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DOI：
10.1016/0306-4522(96)00082-6
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发表时间：
1996-08-01
期刊：
影响因子：
3.3
通讯作者：
Hannibal, J
中科院分区：
文献类型：
--
作者：
Fahrenkrug, J;Hannibal, J
腺苷酸环化酶激活肽（PACAP）是一种新的血管活性肠肽样肽，存在于许多外周器官的神经元成分中。PACAP以两种形式存在，PACAP-27和C-末端延伸的PACAP-38，两者均衍生自相同的前体，其另外产生结构相关的肽，PACAP相关肽。使用特定的放射免疫测定PACAP-38，PACAP-27和PACAP相关肽，我们发现，三个PACAP前体衍生肽存在于大鼠雌性生殖道的所有区域的组织提取物。PACAP-38是主要肽，在输卵管和卵巢中浓度最高。经反相高压液相色谱分析，发现免疫反应物质分别与合成的PACAP-38、PACAP-27和PACAP相关肽共沉淀。免疫组化显示PACAP位于曲张的神经纤维中，与血管、平滑肌和上皮细胞相连。在局部的副交感神经节PACAP免疫反应纤维分支，往往形成静脉曲张，周围的非PACAP阳性细胞体的细胞周丛。此外，成束的PACAP免疫反应纤维横穿神经节。在正常大鼠的海马旁神经节中，只有少数神经元胞体显示PACAP免疫染色，但局部秋水仙碱处理后，出现了中等数量的阳性胞体。原位杂交组织化学与地高辛标记的cRNA探针证实了PACAP的合成在旁神经节的神经元。PACAP和血管活性肠多肽的双重免疫染色揭示了两种肽在大鼠雌性生殖道中的所有管状器官的神经纤维中以及在盲肠旁神经节中的细胞体和神经纤维中的部分共存。新生儿辣椒素治疗后，免疫反应PACAP-38的浓度以及PACAP阳性神经纤维的数量和强度减少，而血管活性肠多肽免疫反应不受影响。总之，PACAP免疫反应神经纤维已被证明在所有地区的大鼠女性生殖道与血管，平滑肌和上皮。在一些纤维，这似乎起源于当地的旁神经节，PACAP与血管活性肠多肽共定位。从这些纤维中释放的PACAP可以单独或与血管活性肠肽一起直接作用于肌肉系统和血管系统，在雌性生殖器官的神经调节中发挥作用。其他含有PACAP的纤维本质上是感觉的，其中一些可能会影响当地的副骶神经节中的神经节传递。版权所有（C）1996 IBRO。爱思唯尔科技有限公司出版
Pituitary adenylate cyclase activating peptide (PACAP) is a novel vasoactive intestinal polypeptide-like peptide which is present in neuronal elements of a number of peripheral organs. PACAP occurs in two forms, PACAP-27 and the C-terminally extended PACAP-38, both derived from the same precursor which in addition gives rise to a structurally-related peptide, PACAP-related peptide. Using specific radioimmunoassays for PACAP-38, PACAP-27 and PACAP-related peptide we found that the three PACAP-precursor-derived peptides were present in tissue extracts from all regions of the rat female genital tract. PACAP-38 was the dominating peptide with the highest concentrations in the Fallopian tribe and the ovary. Upon reverse phase high pressure liquid chromatography the immunoreactive material was found to co-elute with synthetic PACAP-38, PACAP-27 and PACAP-related peptide, respectively. By immunohistochemistry, PACAP was shown to be located in varicose nerve fibres associated with blood vessels, smooth muscle and epithelial cells. Within the local paracervical ganglion PACAP-immunoreactive fibres ramified often forming varicose, pericellular plexuses around non-PACAP-positive cell bodies. Also bundles of PACAP-immunoreactive fibres were transversing the ganglion. In the paracervical ganglion of normal rat only a few neuronal cell bodies showed immunostaining for PACAP, but after local colchichine-treatment a moderate number of positive perikarya appeared. The synthesis of PACAP in neurons of the paracervical ganglia was confirmed by in situ hybridization histochemistry with a digoxigenin-labelled cRNA probe. Double immunostaining for PACAP and vasoactive intestinal polypeptide disclosed a partial co-existence of the two peptides in nerve fibres of all tubular organs in the rat female genital tract and in cell bodies and nerve fibres in the paracervical ganglion. After neonatal capsaicin treatment the concentration of immunoreactive PACAP-38 as well as the number and intensity of PACAP-positive nerve fibres were reduced while vasoactive intestinal polypeptide immunoreactivity was unaffected.In conclusion, PACAP-immunoreactive nerve fibres have been demonstrated in all regions of the rat female genital tract associated with blood vessels, smooth musculature and epithelium. In some fibres, which seem to originate in the local paracervical ganglia, PACAP was co-localized with vasoactive intestinal polypeptide. PACAP released from these fibres could alone or in concert with vasoactive intestinal polypeptide play a role in neuroregulation of female reproductive organs acting directly on the musculature and vasculature. Other PACAP-containing fibres are sensory in nature, and some of these might influence ganglionic neurotransmission in the local paracervical ganglia. Copyright (C) 1996 IBRO. Published by Elsevier Science Ltd.