DMY gene induces male development in genetically female (XX) medaka fish
DMY gene induces male development in genetically female (XX) medaka fish
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DOI：
10.1073/pnas.0611707104
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发表时间：
2007-03-06
影响因子：
11.1
通讯作者：
Nagahama, Yoshitaka
中科院分区：
文献类型：
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作者：
Matsuda, Masaru;Shinomiya, Ai;Nagahama, Yoshitaka
虽然性别决定基因SRY/Sry已在哺乳动物中被鉴定，但在非哺乳类脊椎动物中具有类似功能的同源物和基因尚未被鉴定。最近，DMY（Y染色体上的DM结构域基因）从硬骨鱼青（Oryzias latipes）的Y染色体上的性别决定区克隆。DMY已被证明是男性个体正常发育所必需的。在这项研究中，我们表明，一个117 kb的基因组DNA片段，携带DMY是能够诱导睾丸分化和随后的雄性发育XX（遗传雌性）青鳉。此外，CMV启动子控制下的DMY cDNA的过表达也引起XX性逆转。这些结果表明，DMY是足够的青鳉雄性发育，并建议青鳉的X和Y染色体之间的功能差异是一个单一的基因。我们的数据表明，DMY是脊椎动物中的一个额外的性别决定基因。
Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of the teleost fish medaka (Oryzias latipes). DMY has been shown to be required for the normal development of male individuals. In this study, we show that a 117-kb genomic DNA fragment that carries DMY is able to induce testis differentiation and subsequent male development in XX (genetically female) medaka. In addition, overexpression of DMY cDNA under the control of the CMV promoter also caused XX sex reversal. These results demonstrate that DMY is sufficient for male development in medaka and suggest that the functional difference between the X and Y chromosomes in medaka is a single gene. Our data indicate that DMY is an additional sex-determining gene in vertebrates.