A novel manganese starvation-inducible expression system for Lactobacillus plantarum
A novel manganese starvation-inducible expression system for Lactobacillus plantarum
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DOI：
10.1111/1574-6968.12105
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发表时间：
2013-05-01
影响因子：
2.1
通讯作者：
Fischer, Lutz
中科院分区：
文献类型：
--
作者：
Boehmer, Nico;Koenig, Saskia;Fischer, Lutz
建立了一种新的植物乳杆菌表达系统。该系统是基于首次克隆的植物乳杆菌NC 8锰转运蛋白锰饥饿诱导启动子构建的。利用MRS培养基和pmntH 2-CelB表达载体在低锰浓度下培养植物乳杆菌NC 8，实现了来自激烈火球菌（Pyrococcus furiosus，CelB）的α-葡萄糖苷酶的表达。在生物反应器（4L）中产生8.52katoNPGalL1的CelB活性。新表达系统的优点是不需要添加外部诱导剂，并且另外，不需要进一步引入调控基因。新的启动子符合食品级表达系统的一般要求。
A novel expression system for Lactobacillus plantarum was developed. This system is based on the manganese starvation-inducible promoter from specific manganese transporter of L.plantarum NC8, which was cloned for the first time. The expression of a -glucosidase from Pyrococcus furiosus (CelB) was achieved by cultivating L.plantarum NC8 at low manganese concentrations with MRS medium and the pmntH2-CelB expression vector. A CelB activity of 8.52katoNPGalL1 was produced in a bioreactor (4L). The advantages of the novel expression system are that no addition of an external inducing agent was required, and additionally, no further introduction of regulatory genes was necessary. The new promoter meets the general demands of a food-grade expression system.