POLYAMINES AND POLYAMINE METABOLIZING ENZYME-ACTIVITIES IN HUMAN SEMEN
POLYAMINES AND POLYAMINE METABOLIZING ENZYME-ACTIVITIES IN HUMAN SEMEN
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DOI：
10.1016/0009-8981(73)90418-x
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发表时间：
1973-01-01
影响因子：
5
通讯作者：
ELFVING, K
中科院分区：
文献类型：
--
作者：
JANNE, J;HOLTTA, E;ELFVING, K
本文分析了正常和病理精液中多胺、腐胺、亚精胺和精胺的含量以及多胺代谢酶的活性。在精液分析中未显示明显异常的精液样本的精液含有约0.2 mM腐胺、0.1 mM亚精胺和3 mM精胺。发现精浆是二胺氧化酶活性的异常丰富的来源（EC 1.4.3.6）。此外，多胺合成酶的活动，S-腺苷甲硫氨酸脱羧酶和亚精胺合酶，总是发现在精浆。精子中5-腺苷甲硫氨酸脱羧酶和亚精胺合酶的活性很低或检测不到，但在精子的细胞组分中也发现了确定的二胺氧化酶活性，在低精子密度（小于20 × 106个精子/ml）的精子样品中，二胺氧化酶的活性，特别是亚精胺合酶的活性相对较高。随着精子数的增加，二胺氧化酶和亚精胺合成酶的活性略有下降，但当精子数超过100 × 106/ml时，二胺氧化酶和亚精胺合成酶的活性急剧上升。精浆中精胺的浓度与精液中精子数量的关系只有微小的变化，这种变化与酶活性的变化大致相反。精浆中腐胺浓度与二胺氧化酶活性呈显著负相关，人精浆中二胺氧化酶的某些性质与其它哺乳动物的二胺氧化酶相当。以放射性腐胺为底物，加入未标记的组胺、尸胺、亚精胺和精胺可抑制酶活性。该酶也强烈抑制各种羰基试剂和甲基乙二醛双-（鸟苷酰腙）。
The content of polyamines putrescine, spermidine and spermine as well as the activities of some polyamine-metabolizing enzymes have been analyzed from normal and pathological human semen samples. Seminal fluid from semen samples showing no apparent abnormalities in semen analyses contained about 0.2 mM of putrescine, 0.1 mM of spermidine and 3 mM of spermine. Seminal plasma was found to be an exceptionally rich source of diamine oxidase activity (EC 1.4.3.6). In addition, polyamine-synthesizing enzyme activities,S-adenosylmethionine decarboxylase and spermidine synthase, were invariably found in seminal plasma. Spermatozoa contained very low or undetectable activities of 5-adenosylmethionine decarboxylase and spermidine synthase; however, a definitive diamine oxidase activity was found also in the cellular component of the semen.The activity of diamine oxidase, and especially that of spermidine synthase, was relatively high in semen samples having low sperm density (less than 20 × 106spermatozoa per ml). With increasing number of spermatozoa there was a slight decrease in both activities, but as the sperm count exceeded 100 × 106per ml the activity of diamine oxidase and spermidine synthase sharply increased. The concentration of spermine in seminal plasma showed only minor changes in relation to the number of spermatozoa in semen, the changes being roughly opposite as compared to the changes of the enzyme activities. A significant negative correlation was found between the concentration of putrescine and the activity of diamine oxidase in seminal plasma.Several properties of diamine oxidase from human seminal plasma were found to be comparable to those of other mammalian diamine oxidases. Using radioactive putrescine as the substrate the enzyme activity was inhibited by addition of unlabelled histamine, cadaverine, spermidine and spermine. The enzyme was also powerfully inhibited by various carbonyl reagents and methylglyoxal bis-(guanylhydrazone).