Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients
Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients
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DOI：
10.1021/acs.jproteome.0c00280
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发表时间：
2020-11-06
影响因子：
4.4
通讯作者：
Sinz, Andrea
中科院分区：
文献类型：
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作者：
Ihling, Christian;Taenzler, Dirk;Sinz, Andrea
质谱（MS）可以提供有价值的诊断数据，补充基因组信息，并使我们能够增加我们目前对SARS-CoV-2病毒引起的COVID-19疾病的了解。我们开发了一种简单的基于MS的方法，用于特异性检测COVID-19患者漱口液样本中的SARS-CoV-2蛋白。该方案包括含漱液中所含蛋白质的丙酮沉淀和胰蛋白酶消化，然后进行靶向MS分析。我们的方法鉴定了源自SARS-CoV-2核蛋白的独特肽。在这些有希望的初步结果的基础上，现在可以开发更快的MS方案作为COVID-19患者的常规诊断工具。数据可通过ProteomeXchange获得，标识符为PXD 019423。
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.