Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation
Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation
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DOI：
10.1002/1873-3468.12247
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发表时间：
2016-07
期刊：
影响因子：
3.5
通讯作者：
Rie Miyano;Takashi Matsumoto;H. Takatsu;K. Nakayama;Hye-Won Shin
中科院分区：
文献类型：
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作者：
Rie Miyano;Takashi Matsumoto;H. Takatsu;K. Nakayama;Hye-Won Shin
我们之前已经证明，P4-ATP酶、ATP 10A/ATP 8B 1和ATP 11 A/ATP 11 C分别对磷脂酰胆碱（PC）和氨基磷脂[磷脂酰丝氨酸（PS）和磷脂酰乙醇胺]具有翻转酶活性。在这里，我们研究了PC特异性翻转酶与氨基磷脂特异性翻转酶在细胞外基质上的细胞铺展中的作用。表达PC翻转酶，但不表达PS翻转酶，延迟细胞粘附、细胞铺展并抑制粘着斑的形成。此外，在胞质小叶中隔离PS的PS结合探针的过表达延迟了细胞铺展并抑制了粘着斑的形成。这些结果表明，通过表达PC翻转酶升高质膜细胞质小叶的PC可能会降低PS或磷酸肌醇的局部浓度，这是有效细胞粘附、粘着斑形成和细胞铺展所需的。
We previously showed that P4‐ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC‐specific flippases versus aminophospholipid‐specific flippases in cell spreading on the extracellular matrix. Expression of PC‐flippases, but not PS‐flippases, delayed cell adhesion, cell spreading and inhibited formation of focal adhesions. In addition, overexpression of a PS‐binding probe that sequesters PS in the cytoplasmic leaflet delayed cell spreading and inhibited formation of focal adhesions. These results suggest that elevation of PC at the cytoplasmic leaflet of the plasma membrane by expression of PC‐flippases may reduce the local concentration of PS or phosphoinositides, required for efficient cell adhesion, focal adhesion formation, and cell spreading.