Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
复制标题
神经支配对培养的肌肉细胞上乙酰胆碱受体分布的影响。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
E. Zorychta
中科院分区：
文献类型：
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作者：
M. Anderson;M. Cohen;E. Zorychta
1.将非洲爪蟾胚胎的肌节肌细胞单独或与同一胚胎的神经管细胞一起单层培养。2.电刺激神经胞体引起的自发性抽搐和收缩仅在神经接触的肌细胞中观察到，并且可以被箭毒或α-银环蛇毒素消除。3.在培养的2天内，未与神经接触的肌肉细胞产生一个或多个离散的乙酰胆碱（ACh）受体斑块，如用α-银环蛇毒素的荧光缀合物染色所示。在多聚甲醛固定后进行染色时也观察到类似的斑块，表明它们不是由染料毒素缀合物诱导的。4.用[125 I] α银环蛇毒素标记后的放射自显影显示，斑块的颗粒密度约大于细胞的其余部分。5.受神经支配的细胞上的荧光染色仅限于神经-肌肉接触的路径，有时延伸的长度大于非接触肌肉细胞上观察到的最大斑块。6.当培养物在高浓度的箭毒和卡巴胆碱中生长时，观察到与神经-肌肉接触相关的类似长带染色，这可以防止自发性抽搐。在延迟2 - 3天加入神经管细胞的培养物中也观察到了它们。7.可以得出结论，神经支配导致受体在神经肌肉接触部位积聚，并且该过程可以独立于肌肉活动而运行。
1. Myotomal muscle cells from embryos of Xenopus laevis were cultured as a monolayer either alone or together with neural tube cells from the same embryos. 2. Spontaneous twitching and contractions evoked by electrical stimulation of neural perikarya were observed only in nerve‐contacted muscle cells, and could be abolished by curare or alpha‐bungarotoxin. 3. Within 2 days in culture muscle cells not contacted by nerve developed one or more discrete patches of acetylcholine (ACh) receptors as revealed by staining with fluorescent conjugates of alpha‐bungarotoxin. Similar patches were also seen when staining was carried out after paraformaldehyde fixation, suggesting that they were not induced by the dyetoxin conjugate. 4. Radioautography after labelling with [125I]alpha‐bungarotoxin revealed patches with grain densities approximately twenty‐five‐old greater than over the remainder of the cell. 5. Fluorescent stain on innervated cells was restricted to the path of nerve‐muscle contact and sometimes extended for greater lengths than the largest patches seen on non‐contacted muscle cells. 6. Similar long bands of stain associated with nerve‐‐muscle contacts were observed when cultures were grown in high concentrations of curare and carbachol which prevented spontaneous twitching. They were also seen in cultures in which the addition of neural tube cells was delayed for 2‐3 days. 7. It is concluded that innervation caused receptors to accumulate at sites of nerve‐muscle contact and that this process can operate independently of muscle activity.