Quantitative Proteomics Reveals the Role of Protein Phosphorylation in Rice Embryos during Early Stages of Germination
Quantitative Proteomics Reveals the Role of Protein Phosphorylation in Rice Embryos during Early Stages of Germination
复制标题
定量蛋白质组学揭示蛋白质磷酸化在水稻胚发芽早期的作用
DOI：
10.1021/pr401295c
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Komatsu, Setsuko
中科院分区：
文献类型：
--
作者：
Han, Chao;Yang, Pingfang;Komatsu, Setsuko
种子发芽开始于吸水，结束于胚根出现。使用无凝胶磷酸化蛋白质组学技术研究蛋白质磷酸化事件在水稻种子萌发早期的作用。在吸胀后的前 24 小时内，种子重量和 ATP 含量逐渐增加。蛋白质组学分析表明，碳水化合物代谢和蛋白质合成/降解相关蛋白质主要增加并表现出表达的时间模式。簇和蛋白质-蛋白质相互作用的分析表明，蔗糖合酶和α-淀粉酶的调节是控制发芽的中心事件。磷酸化蛋白质组学分析鉴定了几种参与蛋白质修饰和转录调节的蛋白质，这些蛋白质在发芽过程中磷酸化水平表现出显着的时间变化。聚类分析表明，12种与蛋白质修饰相关的蛋白质在吸胀后12小时时磷蛋白丰度达到峰值。这些结果表明，吸胀后的前 12 小时是水稻种子萌发启动的潜在重要信号转导阶段。参与油菜素类固醇信号转导的三个核心成分在发芽早期阶段磷蛋白丰度显着增加。油菜素内酯处理提高了水稻种子的发芽率，但没有提高胚轴伸长率。这些发现表明油菜素类固醇信号转导可能触发种子发芽。
Seed germination begins with water uptake and ends with radicle emergence. A gel-free phosphoproteomic technique was used to investigate the role of protein phosphorylation events in the early stages of rice seed germination. Both seed weight and ATP content increased gradually during the first 24 h following imbibition. Proteomic analysis indicated that carbohydrate metabolism- and protein synthesis/degradation-related proteins were predominantly increased and displayed temporal patterns of expression. Analyses of cluster and protein-protein interactions indicated that the regulation of sucrose synthases and alpha-amylases was the central event controlling germination. Phosphoproteomic analysis identified several proteins involved in protein modification and transcriptional regulation that exhibited significantly temporal changes in phosphorylation levels during germination. Cluster analysis indicated that 12 protein modification-related proteins had a peak abundance of phosphoproteins at 12 h after imbibition. These results suggest that the first 12 h following imbibition is a potentially important signal transduction phase for the initiation of rice seed germination. Three core components involved in brassinosteroid signal transduction displayed significant increases in phosphoprotein abundance during the early stages of germination. Brassinolide treatment increased the rice seed germination rate but not the rate of embryonic axis elongation. These findings suggest that brassinosteroid signal transduction likely triggers seed germination.