Intra-epithelial mast cells in human airway epithelium: evidence for smoking-induced changes in their frequency.
Intra-epithelial mast cells in human airway epithelium: evidence for smoking-induced changes in their frequency.
复制标题
人气道上皮中的上皮内肥大细胞：吸烟引起其频率变化的证据。
作者：
David Lamb;Alan Lumsden
本文对20例肺癌手术切除的人远侧气道呼吸上皮进行了形态学和定量研究。使用甲苯胺蓝染色的1微米araldite切片进行光学显微镜检查，并在需要时进行进一步的电子显微镜研究，我们描述了细支气管、终末细支气管和呼吸性细支气管上皮中的肥大细胞。这些细胞在两个显微镜水平上的检查表明肥大细胞的许多典型特征-即，在光镜水平上用甲苯胺蓝染色的异染颗粒，表面微绒毛突起，以及在电子显微镜下具有螺旋状亚结构的许多致密的胞浆内颗粒。此外，我们已经确定了细胞缺乏颗粒物质，这可能是公认的肥大细胞凭借其核和细胞表面的特性-发生的光谱之间的变化，这些细胞缺乏颗粒物质和那些与完整的颗粒内容物;和一些细胞中，我们发现了典型的致密，轮状颗粒和大的透明空泡没有这样的材料。通过光学显微镜对所有呼吸道上皮细胞的定量显示肥大细胞占总细胞群的0-2%。吸烟者上皮中肥大细胞的比例高于非吸烟者。由于存在许多上皮肥大细胞缺乏颗粒内容物，我们认为，使用传统的4微米石蜡切片染色噻嗪染料研究肥大细胞将导致显着低估的上皮人口。
A morphological and quantitative study was performed on the respiratory epithelium of human distal airways in 20 lungs removed at operation for the treatment of carcinoma. Using 1 micron araldite sections stained with Toluidine Blue for light microscopy, with further electron microscopic study where required, we have described mast cells in the epithelium of bronchioles, terminal bronchioles, and respiratory bronchioles. Examination of these cells at both microscopic levels demonstrates many of the typical features of mast cells--namely, metachromatic granules on staining with toluidine blue at light microscopic level, surface microvillous processes, and numerous dense intracytoplasmic granules with a whorled substructure at electron microscopy. Furthermore, we have identified cells devoid of granular material which may be recognised as mast cells by virtue of their nuclear and cell surface characteristics--the occurrence of a spectrum of change between those cells devoid of granular material and those with full granule content; and a few cells in which we found both typical dense, whorled granules and large lucent vacuoles devoid of such material. Quantitation of all respiratory epithelial cells by light microscopy shows that mast cells comprise 0-2% of the total population. A higher proportion of mast cells was found in the epithelium of smokers than in non-smokers. Because of the presence of many epithelial mast cells devoid of granule content, we suggest that the use of traditional 4 microns paraffin sections stained with thiazine dyes to study mast cells would lead to a marked underestimation of the epithelial population.