The crystal structure of d(GTACGTAC) at 2.25 A resolution: are the A-DNA's always unwound approximately 10 degrees at the C-G steps?
The crystal structure of d(GTACGTAC) at 2.25 A resolution: are the A-DNA's always unwound approximately 10 degrees at the C-G steps?
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2.25 A 分辨率下 d(GTACGTAC) 的晶体结构：A-DNA 在 C-G 步长上总是展开约 10 度吗？
DOI：
10.1080/07391102.1990.10508524
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发表时间：
1990
影响因子：
4.4
通讯作者：
Takusagawa,F
中科院分区：
文献类型：
--
作者：
Takusagawa,F
The structure of the self-complementary octamer d(GTACGTAC) has been analyzed by a single crystal X-ray diffraction method at 2.25 Å resolution. The crystallographic R factor was 0.184 for all 1233 reflections at this resolution. In spite of the alternating purine-pyrimidine sequence, d(GTACGTAC) adopts the A-form conformation rather than the left-handed Z-form. The average helix twist and the mean rise per base pair are 32.1° and 3.18 Å, respectively. The d(GTACGTAC) helix is characterized by a wide open major groove and small base-pair tilt (9.7°) The partial unwinding of the helix is observed only at the central pyrimidine-purine C-G step, but not at the other pyrimidine-purine T-A steps. Based on this study and six other X-ray studies, we propose a hypothesis that the A-DNA's are always unwound approximately 10° at the C-G steps. Significant differences in base-pair stacking modes are seen between the purine-pyrimidine step and the pyrimidine-purine step. All deoxyribose rings adopt the C3′- endo conformation. All backbone torsion angles fall into the range expected for the A-DNA form, except for the nucleotide G5, whose α and γ torsion angles adopt thetrans, transconformation instead of the commongauche−,gauche+conformation.
