Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling
Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling
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DOI：
10.1016/s0896-6273(02)00751-1
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发表时间：
2002-07-18
期刊：
影响因子：
16.2
通讯作者：
Wilson, SW
中科院分区：
文献类型：
--
作者：
Houart, C;Caneparo, L;Wilson, SW
神经板（ANB）前边界的细胞移植到更后的区域时，可以诱导端脑基因表达。在这里，我们发现了一种分泌的与frizzled相关的Wnt拮抗剂Tic，它在ANB细胞中表达，并能以浓度依赖的方式非自主地促进端脑基因的表达。此外，Tlc功能的缺失会损害端脑发育。我们还发现Wnt8b是前神经板区域命运的局部作用调节剂，可能是Tic拮抗的靶点。最后，我们发现tlc的表达受建立早期前-后和背-腹外胚层模式的信号调控。从这些研究中，我们提出需要前外胚层内Wnt活性的局部拮抗来建立端脑。
Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tic, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tic. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.