Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer
Quantification of free circulating tumor DNA as a diagnostic marker for breast cancer
复制标题
DOI：
10.1089/dna.2008.0744
复制
发表时间：
2008-08-01
影响因子：
3.1
通讯作者：
Medeiros, Rui
中科院分区：
文献类型：
--
作者：
Catarino, Raquel;Ferreira, Maria M.;Medeiros, Rui
目的：通过实时荧光定量PCR方法检测乳腺癌患者和健康人外周血循环DNA的含量，以确定其是否能够区分乳腺癌患者和健康人。研究方法：我们的标准方案，用于定量的无细胞血浆DNA涉及175个连续的乳腺癌患者和80个健康对照。结果：我们发现与对照个体相比，乳腺癌患者的循环DNA水平增加（105.2对77.06 ng/mL，p < 0.001）。我们还发现乳腺手术前后患者的循环DNA量存在统计学显著差异（105.2 vs. 59.0 ng/mL，p 0.001）。血浆游离DNA浓度增加是乳腺癌的一个强危险因素，增加了患乳腺癌的风险（OR，12.32; 95%CI，2.09-52.28; p < 0.001）。结论：通过实时PCR定量循环DNA可能是检测乳腺癌的一个很好的和简单的工具，具有潜在的临床适用性与其他当前用于监测疾病的方法一起。
Aim: To determine whether the amounts of circulating DNA could discriminate between breast cancer patients and healthy individuals by using real-time PCR quantification methodology. Methods: Our standard protocol for quantification of cell-free plasma DNA involved 175 consecutive patients with breast cancer and 80 healthy controls. Results: We found increased levels of circulating DNA in breast cancer patients compared to control individuals (105.2 vs. 77.06 ng/mL, p < 0.001). We also found statistically significant differences in circulating DNA amounts in patients before and after breast surgery ( 105.2 vs. 59.0 ng/mL, p 0.001). Increased plasma cell-free DNA concentration was a strong risk factor for breast cancer, conferring an increased risk for the presence of this disease ( OR, 12.32; 95% CI, 2.09-52.28; p < 0.001). Conclusions: Quantification of circulating DNA by real-time PCR may be a good and simple tool for detection of breast cancer with a potential to clinical applicability together with other current methods used for monitoring the disease.