A deliberate approach to screening for initial crystallization conditions of biological macromolecules
A deliberate approach to screening for initial crystallization conditions of biological macromolecules
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DOI：
10.1016/s1047-8477(03)00048-0
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发表时间：
2003-04-01
影响因子：
3
通讯作者：
DeTitta, GT
中科院分区：
文献类型：
--
作者：
Luft, JR;Collins, RJ;DeTitta, GT
尚未开发出合理预测先前未结晶的大分子的结晶条件的方法。解决这个问题的一种方法是通过广泛撒网，调查大量的化学和物理条件来确定初始结晶条件，以定位结晶引线。详细介绍了一种快速测量结晶铅状态的装置。结晶条件的初始筛选，适用于手动和机器人设置的结果和指导方针进行了讨论。(C)2003年由Elsevier Science（美国）出版。
A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem is to determine initial crystallization conditions by casting a wide net, surveying a large number of chemical and physical conditions to locate crystallization leads. A facility that executes the rapid survey of crystallization lead conditions is described in detail. Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed. (C) 2003 Published by Elsevier Science (USA).