Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity
Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity
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DOI：
10.1016/j.plaphy.2006.10.024
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发表时间：
2006-11-01
影响因子：
6.5
通讯作者：
Zhong, Q.
中科院分区：
文献类型：
--
作者：
Guo, Z.;Ou, W.;Zhong, Q.
比较研究了4个敏感性不同的水稻品种（Oryza sativa L.）抗氧化防御系统对寒冷和干旱胁迫的响应，其中两个品种（香糯1号和紫马糯）耐冷但对干旱敏感，另外两个品种（香中籼2号和IR50）耐干旱但对寒冷敏感。将水稻幼苗转移至生长室中，8℃培养5 d作为低温处理，或28℃作为对照，或28℃但在23％PEG-6000溶液中培养作为干旱胁迫处理。干旱胁迫下湘中县2号的电解质渗漏量、H2O2含量和总硫代巴比妥酸反应物质(TBARS)含量升高。 2号和IR50均低于香诺1号。我和兹玛诺。相反，香诺没有。湘中县1号和紫玛诺号的电解液渗漏、H2O2和TBARS水平明显低于湘中县1号。 2 和 IR50 低温胁迫下。在应激处理过程中测量抗氧化酶（超氧化物歧化酶（SOD）、过氧化氢酶和抗坏血酸过氧化物酶（APX））的活性和抗氧化剂（抗坏血酸和还原型谷胱甘肽）的含量。两个耐旱品种在干旱胁迫后3 d或两个耐冷品种在寒冷胁迫后均显着增强。应激处理后5天它们均下降。另一方面，干旱敏感品种在干旱胁迫后抗氧化酶活性和抗氧化剂含量大幅下降，而冷敏感品种在低温胁迫后抗氧化酶活性和抗氧化剂含量均大幅下降。结果表明，水稻对干旱或寒冷的耐受性与干旱或寒冷条件下抗氧化系统能力的增强密切相关，水稻对干旱或寒冷的敏感性与抗氧化系统能力的下降呈线性相关。 (c) 2006 年 Elsevier Masson SAS。版权所有。
Responses of antioxidative defense systems to chilling and drought stresses were comparatively studied in four cultivars of rice (Oryza sativa L.) differing in sensitivity, two of them (Xiangnuo no. 1 and Zimanuo) are tolerant to chilling but sensitive to drought and the other two (Xiangzhongxian no. 2 and IR50) are tolerant to drought but sensitive to chilling. The seedlings of rice were transferred into growth chamber for 5 d at 8 degrees C as chilling treatment, or at 28 degrees C as control, or at 28 degrees C but cultured in 23% PEG-6000 solution as drought stress treatment. Under drought stress the elevated levels of electrolyte leakage, contents of H2O2 and total thiobarbituric acid-reacting substances (TBARS) in Xiangzhongxian no. 2 and IR50 are lower than those in Xiangnuo no. I and Zimanuo. On the contrary, Xiangnuo no. 1 and Zimanuo have much lower level of electrolyte leakage, H2O2 and TBARS than Xiangzhongxian no. 2 and IR50 under chilling stress. Activities of antioxidant enzymes (superoxide dismutase (SOD), catalase, and ascorbate-peroxidase (APX)) and contents of antioxidants (ascorbaic acid and reduced glutathione) were measured during the stress treatments. All of them were enhanced greatly until 3 d after drought stress in the two drought-tolerant cultivars, or after chilling stress in the two chilling-tolerant cultivars. They all were decreased at 5 d after stress treatments. On the other hand, activities of antioxidant enzymes and contents of antioxidants were decreased greatly in the drought-sensitive cultivars after drought stress, or in the chillingsensitive cultivars after chilling stress. The results indicated that tolerance to drought or chilling in rice is well associated with the enhanced capacity of antioxidative system under drought or chilling condition, and that the sensitivity of rice to drought or chilling is linear correlated to the decreased capacity of antioxidative system. (c) 2006 Elsevier Masson SAS. All rights reserved.