Water channel protein subtype suggests the origin of renal cell carcinoma
Water channel protein subtype suggests the origin of renal cell carcinoma
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DOI：
10.1016/s0022-5347(01)66019-1
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发表时间：
1996-07-01
影响因子：
6.6
通讯作者：
Ikawa, Y
中科院分区：
文献类型：
--
作者：
Kageyama, Y;Sasaki, S;Ikawa, Y
目的：我们克隆了水选择通道蛋白的几种亚型，在正常肾组织中，ChIP-28(28kd的通道形成整合蛋白)仅在近端小管和下行细肢表达，水通道蛋白-CD(Aquaporin-CD，AQP-CD)仅在集合管表达，我们检测了这两种不同类型水通道蛋白在肾癌组织中的表达。材料和方法：采用Northern印迹、逆转录聚合酶链式反应和免疫组织化学方法检测12例原发性肾细胞癌组织中ChIP-28和AQP-CD的表达。用免疫组织化学方法检测4例肾集合管源性Bellini管肿瘤组织中这两种分子的表达。结果：所有肾细胞癌组织中均有CHIP-28的表达，而AQP-CD均不表达。4例Bellini管肿瘤中无1例表达ChIP-28，2例表达AQP-CD。结论：本研究证实了肾细胞癌起源于近端小管或降小管的观点。
Purpose: Several subtypes of water selective channel protein have been cloned, In normal kidneys, CHIP-28 (channel forming integral protein of 28 kd) is expressed solely in the proximal tubules and descending thin limbs, and aquaporin-CD (AQP-CD) expression is restricted to collecting ducts, We assessed expression of these 2 distinct types of water channels in renal cancer tissues.Materials and Methods: Expression of CHIP-28 and AQP-CD was examined in 12 samples of primary renal cell carcinoma by Northern blot, reverse transcriptase-polymerase chain reaction and immunohistochemistry. We also evaluated expression of these 2 molecules immunohistochemically in 4 samples of Bellini duct tumor which has been thought to arise from renal collecting ducts.Results: Expression of CHIP-28 was detected in all of the samples of primary renal cell carcinoma, whereas none of them expressed AQP-CD. None of 4 samples of Bellini duct tumor expressed CHIP-28, although expression of AQP-CD was recognized in 2.Conclusions: Our current observation confirms the idea of the proximal or descending tubule origin of renal cell carcinoma.