Protective effect of forsythiaside A on lipopolysaccharide/D-galactosamine-induced liver injury
Protective effect of forsythiaside A on lipopolysaccharide/D-galactosamine-induced liver injury
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DOI：
10.1016/j.intimp.2015.03.009
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发表时间：
2015-05-01
影响因子：
5.6
通讯作者：
Pan, Zhen-zhen
中科院分区：
文献类型：
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作者：
Pan, Chen-Wei;Zhou, Guang-yao;Pan, Zhen-zhen
连翘苷 A 是从连翘风干果实中分离出来的活性成分，据报道具有多种药理活性，包括抗炎、抗氧化和抗氧化活性。在本研究中，研究了连翘酯苷 A 对脂多糖 (LPS)/D-半乳糖胺 (GalN) 诱导的小鼠急性肝损伤的保肝作用。 LPS(50μg/kg)/GalN(800mg/kg)诱导小鼠急性肝损伤模型。在 LPS/GalN 暴露前 1 小时给予连翘酯苷 A。结果表明，连翘酯苷 A 可减轻 LPS/GalN 诱导的肝脏病理损伤、丙二醛 (MDA) 含量以及血清 ALT 和 AST 水平。此外，连翘酯苷 A 还可抑制 LPS/GalN 诱导的 NF-κ B 激活、血清 TNF-α 和肝脏 TNF-α 水平。此外，我们发现连翘硫苷 A 上调 Nrf2 和血红素加氧酶-1 的表达。我们的结果表明，连翘酯苷 A 通过激活 Nrf2 和抑制 NF-κ B 激活来防止 LPS/GalN 诱导的肝损伤。 (C) 2015 Elsevier B.V. 保留所有权利。
Forsythiaside A, an active constituent isolated from air-dried fruits of Forsythia suspensa, has been reported to have multiple pharmacological activities including anti-inflammatory, anti-oxidant, and antioxidant activities. In the present study, the hepatoprotective effect of forsythiaside A was investigated in lipopolysaccharide (LPS)/D-galactosamine (GalN)-induced acute liver injury in mice. Mice acute liver injury model was induced by LPS (50 mu g/kg)/GalN (800 mg/kg). Forsythiaside A was administrated 1 h prior to LPS/GalN exposure. The results showed that forsythiaside A attenuated hepatic pathological damage, malondialdehyde (MDA) content, and serum ALT, and AST levels induced by LPS/GalN. Moreover, forsythiaside A inhibited NF-kappa B activation, serum TNF-alpha and hepatic TNF-alpha levels induced by LPS/GalN. Furthermore, we found that forsythiaside A upregulated the expression of Nrf2 and heme oxygenase-1. Our results showed that forsythiaside A protected against LPS/GalN-induced liver injury through activation of Nrf2 and inhibition of NF-kappa B activation. (C) 2015 Elsevier B.V. All rights reserved.