Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model
Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model
复制标题
淫羊藿水提取物对去势骨质疏松大鼠模型神经肽信号传导的影响。
DOI：
10.1016/j.jep.2018.04.035
复制
发表时间：
2018-07-15
影响因子：
5.4
通讯作者：
Zhu, Xiaofeng
中科院分区：
文献类型：
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作者：
Liu, Hengrui;Xiong, Yingquan;Zhu, Xiaofeng
民族药理学的相关性：在过去的一千年里，水提取物从淫羊藿（干燥叶淫羊藿brevicornu Maxim。在传统中医中已广泛用于骨病治疗，并据报道在临床试验中显示出对骨质疏松症的有益作用。淫羊藿的治疗作用与中医理论中的脑功能有关。研究目的：确定淫羊藿治疗骨质疏松症与神经肽调节之间的潜在关系。材料与方法：采用高效液相色谱-质谱联用技术对淫羊藿水提液进行了定性定量分析。卵巢切除的大鼠被用作骨质疏松症模型，并在手术后16周用淫羊藿水提取物口服治疗以模拟临床治疗。治疗后，4种神经肽及其主要受体或受体前体的基因表达和蛋白水平，包括：神经肽Y（NPY）及其受体NPY 1（NPYR 1）和2，降钙素基因相关肽及其受体前体降钙素受体样受体（CRLR），血管活性肠肽（VIP）及其受体VIP 1（VIP 1 R）和2，结果：淫羊藿水提物治疗可预防骨质疏松症引起的骨矿物质丢失，减少股骨骨强度下降。神经肽检测显示，治疗还影响了脑/脊髓/骨轴神经肽，特别是治疗增加了脑NPY、骨NPY 1 R、骨CRLR、骨和脊髓VIP和VIP 2 R、骨SP、脑和脊髓NK 1 R。用淫羊藿治疗可以大大减轻骨质疏松症的影响，这很可能是通过与参与调节骨质疏松症的几种神经肽有关的机制。脑/脊髓/骨轴。这些新的结果有助于现有的文献中关于习惯性使用淫羊藿治疗骨质疏松症的可能机制。
Ethnopharmacological relevance: For the past millennium, water extract from Epimedium (dried leaves of Epimediwn brevicornu Maxim.) has been widely used for bone disease therapy in traditional Chinese medicine and has been reported to exhibit salutary effects on osteoporosis in clinical trials. The therapeutic effect of Epimedium is associated with the function of the brain in traditional Chinese medicine theory.Study aim: To determine the potential relationship between treating osteoporosis with Epimedium and neuropeptide regulation.Materials and methods: Water extract from Epimedium was qualitatively and quantitatively analyzed with HPLCTOF-MS. Ovariectomized rats were used as an osteoporosis model and were treated orally with water extract from Epimedium 16 weeks after surgery to mimic clinical therapy. After treatment, gene expression and protein levels of four neuropeptides, as well as their main receptors or receptor precursors including; neuropeptide Y (NPY) and its receptors NPY 1 (NPYR1) and 2; calcitonin gene-related peptide and its receptor precursor calcitonin receptor-like receptor (CRLR); vasoactive intestinal peptide (VIP) and its receptor VIP 1 (VIP1R) and 2; and substance P (SP) and its receptor neurokinin 1 receptor (NK1R) were detected in samples taken from bone, brain and spinal cord.Results: Treatment with water extract from Epimedium prevented bone mineral loss and reduced femoral bone strength decline associated with osteoporosis. Detection of neuropeptides showed that treatment also affected neuropeptide in thebrain/spinal cord/bone axis; specifically, treatment increased brain NPY, bone NPY1R, bone CRLR, bone and spinal cord VIP and VIP2R, bone SP, and brain and spinal cord NK1R.Conclusion: The effects of osteoporosis can largely be reduced by treatment with Epimedium most likely through a mechanism associated with several neuropeptides involved in regulation of the brain/spinal cord/bone axis. These novel results contribute to existing literature regarding the possible mechanisms of habitual use of Epimedium in the treatment of osteoporosis.