1H magnetic resonance spectroscopy of nanomelic chicken cartilage:: effect of aggrecan depletion on cartilage T2
1H magnetic resonance spectroscopy of nanomelic chicken cartilage:: effect of aggrecan depletion on cartilage T2
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DOI：
10.1016/s1063-4584(03)00155-9
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发表时间：
2003-10-01
影响因子：
7
通讯作者：
Smith, MB
中科院分区：
文献类型：
--
作者：
Mosher, TJ;Chen, Q;Smith, MB
目的：为了确定蛋白聚糖消耗对软骨质子磁共振（MR）光谱T-2的影响，使用纳米级鸡软骨，一种完全缺乏聚集蛋白聚糖的遗传突变体。设计：使用96回波脉冲序列，在MRI上测量正常胚胎和短肢股骨骺软骨的质子磁共振波谱T-2，回波延迟时间在60 μ s至1.7 s的TE周期内呈几何级数增加。软骨T-2成分的相对强度和分布通过将信号衰减曲线拟合为多指数函数来确定。信号衰减曲线中T-2分量的数量由拟合的自由度限制r（2）确定。对于正常的鸡胚软骨，总信号的97.6 ± 0.2%（平均值± 95%置信区间）包括长T-2分量（179.1 ± 1.3 ms）和相对较小的短T-2分量（0.5 ± 0.4 ms）。短肢软骨的T-2分布更不均匀，确定了四个组分：两个短T-2组分（0.5+/-0.02和7.3+/-0.6 ms），一个大的中间组分（56.4+/-5.6 ms）和一个广泛分布的长组分（137.5+/-16.6 ms）。在nanomelic软骨有更大的异质性的软骨T-2表示更大的变化，在水质子的流动性和交换水与细胞外matrix.Conclusion：在细胞外软骨基质中的聚集蛋白聚糖的缺乏产生更大的异质性的软骨T-2，但不会增加T-2，如先前报道的胶原基质的退行性变化。(C)2003年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: To determine the effect of proteoglycan depletion on cartilage proton magnetic resonance (MR) spectroscopy T-2 using nanomelic chicken cartilage, a genetic mutant that completely lacks aggrecan.Design: Proton MR spectroscopic T-2 measurements of normal embryonic and nanomelic femoral epiphyseal cartilage were obtained using a 96-echo pulse sequence with inter-echo delay times increased logarithmically over the TE period of 60 mus to 1.7 s. The relative intensity and distribution of cartilage T-2 components were determined by fitting signal decay curves to a multi-exponential function. The number of T-2 components in the signal decay curves was determined by the degree of freedom limited r(2) of the fit.Results: For normal fetal chicken cartilage, 97.6+/-0.2% (mean+/-95% confidence interval) of the total signal comprises a long T-2 component (179.1+/-1.3 ms) with a relatively small short T-2 component (0.5+/-0.4 ms). The T-2 distribution for nanomelic cartilage is more heterogeneous with four components identified: two short T-2 Components (0.5+/-0.02 and 7.3+/-0.6 ms), a large intermediate component (56.4+/-5.6 ms), and a broadly distributed long component (137.5+/-16.6 ms). In nanomelic cartilage there is greater heterogeneity of cartilage T-2 indicating greater variation in water proton mobility and exchange of water with the extracellular matrix.Conclusion: Absence of aggrecan in the extracellular cartilage matrix produces greater heterogeneity in cartilage T-2, but will not increase T-2 as has been previously reported with degenerative change of the Collagen matrix. (C) 2003 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.