Expression of vitellogenin and cortical rod proteins during induced ovarian development by eyestalk ablation in the kuruma prawn, Marsupenaeus japonicus
Expression of vitellogenin and cortical rod proteins during induced ovarian development by eyestalk ablation in the kuruma prawn, Marsupenaeus japonicus
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DOI：
10.1016/j.cbpa.2005.12.002
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发表时间：
2006-02-01
影响因子：
2.3
通讯作者：
Aida, K
中科院分区：
文献类型：
--
作者：
Okumura, T;Kim, YK;Aida, K
在对虾类中，卵巢发育的特征是主要卵黄蛋白（卵黄蛋白）的积累和卵母细胞中皮质杆的形成。这个过程被认为是在眼柄中的神经内分泌器官（X器官窦腺复合体）的控制下。在本研究中，卵黄蛋白原（VTG，前体的卵黄蛋白）和两种皮质杆蛋白（皮质杆蛋白，CRP;血小板反应蛋白，MjTSP）的合成诱导双侧眼柄切除（去除X-器官窦腺复合体）在未成熟的女性库鲁马对虾，Marsupenaeus prawn，和合成过程进行了监测超过7天的时间内消融后。卵巢重量和血淋巴VTG水平增加，在消融的女性。切除眼柄后，卵巢中VTG mRNA的表达水平随着卵黄蛋白的积累而增加。另一方面，卵巢中的CRP和MjTSP蛋白水平在眼柄切除后增加，而卵巢中的CRP和MjTSP mRNA水平没有伴随变化。结果表明，眼柄激素对基因表达的调控机制在转录调控（VTG）和翻译调控（CRP-MjTSP）中是不同的。(C)2005年爱思唯尔公司All rights reserved.
In penaeid shrimp species, ovarian development is characterized by the accumulation of a major yolk protein (vitellin) and the formation of cortical rods in the oocytes. The process is considered to be under the control of a neuroendocrine organ in the eyestalk (the X-organ sinus gland complex). In the present study, the synthesis of vitellogenin (VTG, precursor of vitellin) and two kinds of cortical rod proteins (cortical rod protein, CRP; thrombospondin, MjTSP) was induced by bilateral eyestalk ablation (removal of the X-organ sinus gland complex) in immature female kuruma prawn, Marsupenaeus japonicus, and the synthesis process was monitored over a 7-day period after the ablation. The ovarian weight and hemolymph VTG levels increased in the ablated females. The VTG mRNA levels in the ovary increased concomitantly with vitellin accumulation in the ovary after eyestalk ablation. On the other hand, the CRP and MjTSP protein levels in the ovary increased after eyestalk ablation, whereas the CRP and MjTSP mRNA levels in the ovary did not change concomitantly. The results suggest that the regulatory mechanism of gene expression by eyestalk hormones is different between VTG (transcriptional control) and CRP-MjTSP (translational control). (C) 2005 Elsevier Inc. All rights reserved.