Enhanced bio-molecular interactions through recirculating microflows
Enhanced bio-molecular interactions through recirculating microflows
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DOI：
10.1049/iet-nbt:20070029
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发表时间：
2008-06-01
影响因子：
2.3
通讯作者：
Packirisamy, M.
中科院分区：
文献类型：
--
作者：
Chandrasekaran, A.;Packirisamy, M.
已开发出用于进行光学生物检测的循环微流体平台。通过适当的设计和流量控制，本装置可用于生物物种与微流体通道的被动混合而无需固定。使用本装置进行生物检测的可行性已通过荧光方法得到证明，并且实验是使用带有 Alexa Fluor 647 (AF647) 荧光团颗粒标记的抗羊抗体 (AB) 进行的。通过控制流体流量，可以将AB单独隔离到微流体通道内的再循环区域中，从而实现定性和定量生物检测。已经对流动行为进行了有限元建模，结果与使用标记抗体颗粒获得的流动可视化结果相似。因此，本工作为使用混合集成装置在基于荧光的化学和生物传感中对生物对或单个样本进行原位快速生物医学检测提供了信心。
A recirculating microfluidic platform has been developed for carrying out optical bio-detection. The present device can be used for passive mixing of the biological species with the microfluidic channel without immobilisation, through appropriate design and flow control. The feasibility of bio-detection using the present setup has been demonstrated through the method of fluorescence and the experiments were carried out with Antisheep Antibody (AB) tagged with Alexafluor 647 (AF647) fluorophore particles. By controlling the fluid flow, it was possible to isolate AB separately into a recirculation zone within the microfluidic channel, thereby enabling qualitative and quantitative bio-detection. Finite element modelling of the flow behaviour has been carried out and the results were similar to the results of flow visualisation obtained with tagged antibody particles. The present work thus provides confidence in using the hybrid integrated device for in situ rapid biomedical detection of biological pairs or individual specimen in fluorescence-based chemical and biological sensing.