Neonatal bacillus Calmette-Guerin vaccination inhibits de novo allergic inflammatory response in mice via alteration of CD4+CD25+ T-regulatory cells
Neonatal bacillus Calmette-Guerin vaccination inhibits de novo allergic inflammatory response in mice via alteration of CD4+CD25+ T-regulatory cells
复制标题
DOI：
10.1038/aps.2008.3
复制
发表时间：
2009-01-01
影响因子：
8.2
通讯作者：
Shen, Hua-hao
中科院分区：
文献类型：
--
作者：
Li, Qian;Shen, Hua-hao
目的：卫生学假说表明，缺乏细菌感染将有利于过敏性疾病的发展。牛分枝杆菌卡介苗(BCG)感染可抑制变应原诱导的哮喘反应，但其对免疫反应的潜在机制尚不清楚。T调节细胞(Treg)被认为是一种重要的免疫调节细胞，能够调节获得性免疫反应。本研究旨在探讨卡介苗对过敏性肺部炎症的保护作用是否与哮喘小鼠外周血中CD4(+)CD25(+)Treg细胞的变化有关，并探讨Treg细胞的作用机制。观察嗜酸性粒细胞的浸润情况。检测小鼠脾组织中CD4(+)、CD25(+)Treg细胞的百分率和特异性转录因子Foxp3的表达。检测细胞毒性淋巴细胞相关抗原(CTLA)-4的表达及细胞因子白介素10(IL-10)和转化生长因子-β(TGF-β)的水平。结果：卡介苗治疗可抑制哮喘小鼠的从头变态反应性炎症反应。卡介苗治疗后CD4+CD25+Treg细胞和Foxp3表达增加，CTLA-4表达增加，细胞因子IL-10和TGF-β水平升高(P
Aim: The hygiene hypothesis suggests a lack of bacterial infections would favor the development of allergic diseases. Mycobacterium bovis bacille Calmette-Guerin (BCG) infection can inhibit allergen-induced asthma reactions, but the underlying mechanism of this infection on the immunological responses is unclear. T-regulatory (Treg) cells are thought to play a role as a crucial immunoregulatory cells that are capable of regulating adaptive immune responses. We conducted this study to investigate whether the protective effect of the BCG vaccination on allergic pulmonary inflammation is associated with the alteration of CD4(+)CD25(+) Treg cells in a murine asthma model and the mechanisms of Treg cells.Methods: Newborn C57BL/6 mice were vaccinated 3 times with BCG on d 0, 7, and 14 and subsequently sensitized and challenged with ovalbumin. Eosinophil infiltration was investigated. The frequencies of spleen CD4(+)CD25(+) Treg cells and the expression of specific transcriptional factor Foxp3 were assayed. The cytotoxic lymphocyte associated antigen (CTLA)-4 expression and cytokine interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta) levels were measured.Results: We showed that treatment of mice with BCG inhibited de novo allergic inflammatory response in a mouse model of asthma. BCG treatments are associated with the increase of CD4(+)CD25(+) Treg cells and Foxp3 expression, accompanied by an increased CTLA-4 expression and cytokine IL-10 and TGF-beta levels (P