Comamonas denitrificans sp nov., an efficient denitrifying bacterium isolated from activated sludge
Comamonas denitrificans sp nov., an efficient denitrifying bacterium isolated from activated sludge
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DOI：
10.1099/00207713-51-3-999
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发表时间：
2001-05-01
影响因子：
2.8
通讯作者：
Dalhammar, G
中科院分区：
文献类型：
--
作者：
Gumaelius, L;Magnusson, G;Dalhammar, G
为了寻找活性污泥反硝化的生物标志物，对从3个污水处理厂分离的5株反硝化菌进行了研究。这些菌株是从1500个菌株中选出的，因为它们具有优良的发酵性能。当从好氧条件转换到缺氧条件时，它们快速分解，没有滞后期。所有菌株都具有cd（1）型亚硝酸盐还原酶。这些菌株是革兰氏阴性杆菌，并且当在液体溶液中培养时它们都以丝状链生长。当在营养琼脂上生长时，菌株的菌落形态不同。测定了几乎全长的16 S rDNA序列，系统发育分析显示这些菌株位于变形菌门β亚类中丛毛单胞菌属的成员之间。还分析了签名核苷酸和自举百分比以验证该位置。根据16 SrDNA序列分析，菌株110、123（T）、2.99g、5.38g和P17与已知菌株的相似性小于或等于96.7%，但彼此之间的相似性大于或等于99.7%，在系统发育树中紧密聚在一起。在16 S rRNA基因的序列基序也被发现，这表明这些菌株的单系起源。然而，一些菌株与其他菌株不同，例如菌株110从其他菌株中较早分支，5.38g在表型上更惰性。因此，建议将菌株110、123（T）、2.99g和P17归为一新种，即假单胞菌Comamonasecompicans sp. nov.，5.38g菌株的分类地位有待进一步研究。细菌丛毛单胞菌的模式菌株是123（T）（ATCC 700936（T））。
To find a biomarker for denitrification in activated sludge, five denitrifying strains isolated from three wastewater treatment plants were studied. These strains were selected from among 1500 isolates for their excellent denitrifying properties. They denitrify quickly and have no lag phase when switching from aerobic to anoxic conditions. All strains have the cd(1)-type of nitrite reductase. The strains are Gram-negative rods and they all grow as filamentous chains when cultivated in liquid solution. The strains differ in colony morphology when grown on nutrient agar. Almost full-length 16S rDNA sequences were determined and phylogenetic analysis revealed that these strains are positioned among members of the genus Comamonas in the beta -subclass of the Proteobacteria. Signature nucleotides and bootstrap percentages were also analysed to verify this position. Strains 110, 123(T), 2.99g, 5.38g and P17 were less than or equal to 96.7 % similar to known strains, but greater than or equal to 99.7 % similar to each other, as judged from their 16S rDNA sequences, and grouped tightly together in the phylogenetic tree. Sequence motifs in the 16S rRNA gene were also found, suggesting the monophyletic origin of these strains. Nevertheless, some strains differed from the others, for example strain 110 branches early from the other strains and 5.38g is phenotypically more inert. Therefore, it is proposed that strains 110, 123(T), 2.99g and P17 are classified into a new species, Comamonas denitrificans sp. nov., while the taxonomic status of strain 5.38g will have to await the outcome of further studies. The type strain of Comamonas denitrificans is 123(T) (ATCC 700936(T)).